Recombinant Anti-Pro Caspase-8 antibody [EPR162] (ab108333)
![Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-254043-anti-caspase-8-antibody-epr162-western-blot.jpg "Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR162] to Pro Caspase-8
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Pro Caspase-8 antibody [EPR162]
See all Pro Caspase-8 primary antibodies -
Description
Rabbit monoclonal [EPR162] to Pro Caspase-8 -
Host species
Rabbit -
Specificity
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, IM9, HepG2, HeLa, and HL60 cell lysates; Human tonsil tissue and Human hepatocellular carcinoma tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR162 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Relevance
Disease: Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. -
Database links
- Entrez Gene: 841 Human
- Entrez Gene: 12370 Mouse
- Entrez Gene: 64044 Rat
- Omim: 601763 Human
- SwissProt: Q14790 Human
- SwissProt: O89110 Mouse
- SwissProt: Q9JHX4 Rat
Images
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Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Pro Caspase-8 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108333 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108333 was shown to specifically react with Caspase-8 when Caspase-8 knockout samples were used. Wild-type and Pro Caspase-8 knockout samples were subjected to SDS-PAGE. ab108333 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-282046-anti-caspase-8-antibody-epr162-western-blot.jpg)
Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)All lanes : Anti-Pro Caspase-8 antibody [EPR162] (ab108333) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDaLanes 1 - 3: Merged signal (red and green). Green - ab108333 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108333 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-120911-anti-caspase-8-antibody-epr162-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)ab108333, at 1/100 dilution, staining Pro Caspase-8 in paraffin-embedded human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-279975-anti-caspase-8-antibody-epr162-western-blot.jpg)
Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Pro Caspase-8 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green).Green - target observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
This western blot image is a comparison between ab108333 and a competitor's top cited mouse monoclonal antibody.
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![Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-120913-anti-caspase-8-antibody-epr162-western-blot.jpg)
Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)All lanes : Anti-Pro Caspase-8 antibody [EPR162] (ab108333) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : IM9 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa -
![Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-120912-anti-caspase-8-antibody-epr162-western-blot.jpg)
Western blot - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)All lanes : Anti-Pro Caspase-8 antibody [EPR162] (ab108333) at 1/1000 dilution
Lane 1 : Jurkat cell lysate, treated with etoposide
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate, treated staurosporine
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-120910-anti-caspase-8-antibody-epr162-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pro Caspase-8 antibody [EPR162] (ab108333)ab108333, at 1/100 dilution, staining Pro Caspase-8 in paraffin-embedded human hepatocellular carcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Pro Caspase-8 antibody [EPR162] (ab108333)](https://www.abcam.com/ps/products/108/ab108333/Images/ab108333-10-benefits-of-recombinant-antibodies.png)
Anti-Pro Caspase-8 antibody [EPR162] (ab108333)
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab108333 has been referenced in 15 publications.
- Shi C et al. LncRNA OIP5-AS1 promotes cell proliferation and migration and induces angiogenesis via regulating miR-3163/VEGFA in hepatocellular carcinoma. Cancer Biol Ther 21:604-614 (2020). PubMed: 32329664
- Gao T et al. Magnolol induces human Ewing sarcoma SK-ES-1 cell apoptosis via the mitochondrial and death receptor pathways. Am J Transl Res 12:1672-1682 (2020). PubMed: 32509168
- Wang WM et al. Kupffer Cell-Derived TNF-a Triggers the Apoptosis of Hepatic Stellate Cells through TNF-R1/Caspase 8 due to ER Stress. Biomed Res Int 2020:8035671 (2020). PubMed: 32802876
- Zeng CY et al. ATP induces caspase-3/gasdermin E-mediated pyroptosis in NLRP3 pathway-blocked murine macrophages. Apoptosis 24:703-717 (2019). PubMed: 31175486
- Liao Y et al. Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1ß During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation. Front Immunol 10:268 (2019). PubMed: 30846986
