Overview

  • Product name
    Anti-pro Caspase 9 (phospho T125) antibody
    See all pro Caspase 9 primary antibodies
  • Description
    Rabbit polyclonal to pro Caspase 9 (phospho T125)
  • Host species
    Rabbit
  • Specificity
    ab138412 detects endogenous levels of pro Caspase 9 only when phosphorylated at T125.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide derived from Human pro Caspase 9 around the phosphorylation site of Threonine 125.

  • Positive control
    • HeLa cells treated with TNF at 20 ng/ml for 5 minutes and calyculin A at 50 ng/ml for 5 minutes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab138412 was purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificity
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stage
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
    Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase-9 subunit p10 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • ICELAP6 antibody
    • MCH6 antibody
    see all

Images

  • Anti-pro Caspase 9 (phospho T125) antibody (ab138412) at 1/500 dilution + HeLa cells treated with TNF at 20 ng/ml for 5 minutes and calyculin A at 50 ng/ml for 5 minutes at 30 µg

    Predicted band size: 46 kDa

References

This product has been referenced in:
  • Wang Z  et al. Long noncoding RNA maternally expressed gene 3 knockdown alleviates lipopolysaccharide-induced inflammatory injury by up-regulation of miR-203 in ATDC5 cells. Biomed Pharmacother 100:240-249 (2018). WB . Read more (PubMed: 29432995) »
  • Yu T  et al. Ligustrazine protects chondrocyte against IL-1ß induced injury by regulation of SOX9/NF-?B signaling pathway. J Cell Biochem 119:7419-7430 (2018). Read more (PubMed: 29806884) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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