Overview

  • Product name

    Anti-pro-EGF antibody [EPR14622]
    See all pro-EGF primary antibodies
  • Description

    Rabbit monoclonal [EPR14622] to pro-EGF
  • Host species

    Rabbit
  • Specificity

    Recognises the Pro-epidermal growth factor chain.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human pro-EGF aa 200-350. The exact sequence is proprietary.
    Database link: P01133

  • Positive control

    • WB: Human EGF recombinant protein; Human milk sample; Human urine sample. IP: Human milk sample
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 160 kDa (predicted molecular weight: 134 kDa).
IP 1/40.

Images

  • All lanes : Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

    Lane 1 : Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag
    Lane 2 : Recombinant Human EGF recombinant protein (aa971-1023)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 134 kDa



    Blocking and Diluting buffer and concentration:5% NFDM/TBST

    Exposure time:1 second

  • Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/5000 dilution + Human EGF recombinant protein at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 134 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human EGF full length protein contains aa21-1023 with His-Tag® at the C-Terminus.

  • Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution + Human milk sample at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 134 kDa
    Observed band size: 160 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution + Human urine sample at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 134 kDa
    Observed band size: 160 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • EGF was immunoprecipitated from 1mg of Human milk sample with ab206106 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206106 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human milk sample 10ug (Input).

    Lane 2: ab206106 IP in Human milk sample.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206106 in Human milk sample.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab206106 has not yet been referenced specifically in any publications.

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