Anti-Pro + Mature Surfactant Protein B antibody (ab40876)

Overview

  • Product name
    Anti-Pro + Mature Surfactant Protein B antibody
  • Description
    Rabbit polyclonal to Pro + Mature Surfactant Protein B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Cow, Human
  • Immunogen

    Full length native SP-B protein (purified) (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab40876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 42 kDa. (reduced or non-reduced samples).
ELISA 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • Alternative names
    • PSP B antibody
    • PSPB antibody
    • Pulmonary surfactant apoprotein PSP-B antibody
    • SFTPB antibody
    • SP B antibody
    • SP-B antibody
    • SPB antibody
    • surfactant protein B antibody
    • Surfactant, pulmonary-associated protein B antibody
    see all

Images

  • Adult mouse lung was embedded in paraffin and stained with ab40876 (1/500 dilution).
  • All lanes : Anti-Pro + Mature Surfactant Protein B antibody (ab40876) at 1/5000 dilution

    Lane 1 : Adult mouse lung homogenate
    Lane 2 : Human lung homogenate
    Lane 3 : Surfactant Protein B peptide
    Lane 4 : HEK-293T lysate

    Predicted band size: 42 kDa

References

This product has been referenced in:
  • Bartolák-Suki E  et al. Optimization of Variable Ventilation for Physiology, Immune Response and Surfactant Enhancement in Preterm Lambs. Front Physiol 8:425 (2017). Read more (PubMed: 28690548) »
  • Khoshgoo N  et al. MicroRNA-200b regulates distal airway development by maintaining epithelial integrity. Sci Rep 7:6382 (2017). Read more (PubMed: 28743913) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (amniotic epithelial cells)
Permeabilization
Yes - Permeabilization Buffer
Gating Strategy
Epithelial cells
Specification
amniotic epithelial cells
Fixation
Formaldehyde

Ms. Angela Lemke

Verified customer

Submitted Jan 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Liver [surfactant protein B])
Loading amount
100 µg
Specification
Liver [surfactant protein B]
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. SeongHoon Cho

Verified customer

Submitted Nov 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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