Western blot abreview for Anti-Surfactant Protein B (Mature) antibody

Good
Abreviews
Application
Western blot
Sample
Human Recombinant protein (Liver [surfactant protein B])
Loading amount
100 µg
Specification
Liver [surfactant protein B]
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking[5% Skim milk in TPBS(1%)]

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse IgG(H+L) HRP conjugate
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 50 kDa Non-specific: 15, 40 kDa
Positive control
Non-induced MBP-mature SP-B
Negative control
Induced MBP-mature SP-B

Additional data

PubMed ID
ysbio: View

Dr. SeongHoon Cho

Verified customer

Submitted Nov 12 2009

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