Anti-NRG1 antibody (ab217805)
Key features and details
- Rabbit polyclonal to NRG1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NRG1 antibody
See all NRG1 primary antibodies -
Description
Rabbit polyclonal to NRG1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Pro-neuregulin-1, membrane-bound isoform aa 50-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q02297 -
Positive control
- Rat kidney and mouse brain tissues; Raji cell lysate and mouse brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Predicted molecular weight: 70 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 70 kDa. |
Target
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Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. -
Tissue specificity
Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. -
Involvement in disease
Note=A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with ODZ4. The translocation fuses the 5'-end of ODZ4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines. -
Sequence similarities
Belongs to the neuregulin family.
Contains 1 EGF-like domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Developmental stage
Detectable at early embryonic ages. Isoform 10 is highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons. Type IV isoforms are expressed in fetal brain. -
Domain
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
ERBB receptor binding is elicited entirely by the EGF-like domain. -
Post-translational
modificationsProteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage. -
Cellular localization
Secreted; Cell membrane. Does not seem to be active; Membrane. May possess an internal uncleaved signal sequence; Nucleus. May be nuclear and Secreted. Has a signal peptide. - Information by UniProt
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Database links
- Entrez Gene: 3084 Human
- Entrez Gene: 211323 Mouse
- Entrez Gene: 112400 Rat
- Omim: 142445 Human
- SwissProt: Q02297 Human
- SwissProt: P43322 Rat
- Unigene: 453951 Human
- Unigene: 668810 Human
see all -
Alternative names
- Acetylcholine receptor-inducing activity antibody
- Acetylcholine receptor-inducing activity, CHICK, homolog of antibody
- ARIA antibody
see all
Images
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All lanes : Anti-NRG1 antibody (ab217805) at 1/300 dilution
Lane 1 : mouse brain lysate
Lane 2 : Raji cell lysate
Secondary
All lanes : goat anti-rabbit IgG antibody (H+L), HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa15% gel
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling Pro-neuregulin-1, membrane-bound isoform with ab217805 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Pro-neuregulin-1, membrane-bound isoform with ab217805 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab217805 has not yet been referenced specifically in any publications.