Overview

  • Product name

    Anti-Pro-neuregulin-1, membrane-bound isoform antibody
    See all Pro-neuregulin-1, membrane-bound isoform primary antibodies
  • Description

    Rabbit polyclonal to Pro-neuregulin-1, membrane-bound isoform
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pro-neuregulin-1, membrane-bound isoform aa 50-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q02297

  • Positive control

    • Rat kidney and mouse brain tissues; Raji cell lysate and mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab217805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/100 - 1/1000. Predicted molecular weight: 70 kDa.

Target

  • Function

    Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
  • Tissue specificity

    Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific.
  • Involvement in disease

    A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with TENM4. The translocation fuses the 5'-end of TENM4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines.
  • Sequence similarities

    Belongs to the neuregulin family.
    Contains 1 EGF-like domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage

    Detectable at early embryonic ages. Isoform 10 is highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons. Type IV isoforms are expressed in fetal brain.
  • Domain

    The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • Post-translational
    modifications

    Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
  • Cellular localization

    Secreted; Cell membrane. Does not seem to be active; Membrane. May possess an internal uncleaved signal sequence; Nucleus. May be nuclear and Secreted. Has a signal peptide.
  • Information by UniProt
  • Database links

  • Form

    Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note: Does not seem to be active.
  • Alternative names

    • Acetylcholine receptor-inducing activity antibody
    • Acetylcholine receptor-inducing activity, chick, homolog of antibody
    • ARIA antibody
    • Breast cancer cell differentiation factor p45 antibody
    • GGF antibody
    • GGF2 antibody
    • glial growth factor 2 antibody
    • Glial growth factor antibody
    • Heregulin antibody
    • heregulin, alpha (45kD, ERBB2 p185-activator) antibody
    • heregulin, alpha antibody
    • HGL antibody
    • HRG antibody
    • HRG1 antibody
    • HRGA antibody
    • MST131 antibody
    • MSTP131 antibody
    • NDF antibody
    • Neu differentiation factor antibody
    • Neuregulin-1 antibody
    • nrg1 antibody
    • NRG1-IT2 antibody
    • NRG1_HUMAN antibody
    • Pro-NRG1 antibody
    • Sensory and motor neuron-derived factor antibody
    • SMDF antibody
    see all

Images

  • All lanes : Anti-Pro-neuregulin-1, membrane-bound isoform antibody (ab217805) at 1/300 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : Raji cell lysate

    Secondary
    All lanes : goat anti-rabbit IgG antibody (H+L), HRP-conjugated at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa



    15% gel

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling Pro-neuregulin-1, membrane-bound isoform with ab217805 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Pro-neuregulin-1, membrane-bound isoform with ab217805 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab217805 has not yet been referenced specifically in any publications.

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