Anti-NT-proBNP antibody [15F11] (ab13115)
Key features and details
- Mouse monoclonal [15F11] to NT-proBNP
- Suitable for: ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-NT-proBNP antibody [15F11]
See all NT-proBNP primary antibodies -
Description
Mouse monoclonal [15F11] to NT-proBNP -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human NT-proBNP aa 1-100.
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General notes
Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Chromatography on protein A Sepharose -
Clonality
Monoclonal -
Clone number
15F11 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa. |
Target
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANFB_HUMAN antibody
- BNP(1-32) antibody
- BNP(5-29) antibody
see all
Images
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WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab13115 has been referenced in 6 publications.
- Knight WE et al. A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes. STAR Protoc 2:100912 (2021). PubMed: 34755117
- Karhu ST et al. GATA4-targeted compound exhibits cardioprotective actions against doxorubicin-induced toxicity in vitro and in vivo: establishment of a chronic cardiotoxicity model using human iPSC-derived cardiomyocytes. Arch Toxicol 94:2113-2130 (2020). PubMed: 32185414
- Pohjolainen L et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253
- Mosqueira D et al. High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro. Methods Protoc 2:N/A (2019). PubMed: 31717790
- Nagai H et al. Antihypertrophic Effects of Small Molecules that Maintain Mitochondrial ATP Levels Under Hypoxia. EBioMedicine 24:147-158 (2017). PubMed: 28942281
- Tonne JM et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). IP ; Human . PubMed: 21482747