Western blot abreview for Anti-Natriuretic peptides A antibody

Excellent
Abreviews
Application
Western blot
Sample
Dog Tissue lysate - whole (atrium and ventricle)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
75 µg
Specification
atrium and ventricle
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/250

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: ECL-anti rabbit IgG HRP linked whole antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Super signal (thermo)
Negative control
canine ventricle
Exposure
30 second(s)
Bands
Specific: +/-15 kDa
Positive control
canine atrium

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Submitted Dec 22 2010

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