• Product name

    Anti-proBNP antibody [16F3] (HRP)
    See all proBNP primary antibodies
  • Description

    Mouse monoclonal [16F3] to proBNP (HRP)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: ELISA, WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 13-27 of Human proBNP

  • General notes

    Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.




Our Abpromise guarantee covers the use of ab13124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [50E1] to BNP (ab20984).
WB Use at an assay dependent dilution. Detects a band of approximately 13 kDa.
Sandwich ELISA Use at an assay dependent dilution. Detection


  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities

    Belongs to the natriuretic peptide family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • ANFB_HUMAN antibody
    • BNP antibody
    • BNP(1-32) antibody
    • BNP(5-29) antibody
    • BNP-32 antibody
    • Brain type natriuretic peptide antibody
    • Gamma brain natriuretic peptide antibody
    • Gamma-brain natriuretic peptide antibody
    • Natriuretic peptide precursor B antibody
    • nppb antibody
    • NT proBNP antibody
    see all


  • WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].


This product has been referenced in:

  • Kim YS  et al. High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli. Appl Microbiol Biotechnol 103:4779-4788 (2019). Read more (PubMed: 31041470) »
  • Duan Q  et al. BET bromodomain inhibition suppresses innate inflammatory and profibrotic transcriptional networks in heart failure. Sci Transl Med 9:N/A (2017). Read more (PubMed: 28515341) »
See all 3 Publications for this product

Customer reviews and Q&As


Thank you very much for your inquiry.

I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

AA 1-12 no homology
AA 13-27 58%
AA 28-45 62%
AA 61-76 no homology.

I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up