Anti-proBNP antibody [C1] (ab239514)
![Western blot - Anti-proBNP antibody [C1] (ab239514)](https://www.abcam.com/ps/products/239/ab239514/Images/ab239514-317931-anti-probnp-antibody-c1-western-blot.jpg "Western blot - Anti-proBNP antibody [C1] (ab239514)")
Key features and details
- Mouse monoclonal [C1] to proBNP
- Suitable for: WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG2a
Overview
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Product name
Anti-proBNP antibody [C1]
See all proBNP primary antibodies -
Description
Mouse monoclonal [C1] to proBNP -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Mouse proBNP aa 1-100 conjugated to ovalbumin.
Database link: P40753-1 -
Positive control
- WB: Human serum. Pig and rat heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4879 Human
- Entrez Gene: 18158 Mouse
- Entrez Gene: 25105 Rat
- Omim: 600295 Human
- SwissProt: P16860 Human
- SwissProt: P40753 Mouse
- SwissProt: P13205 Rat
- Unigene: 219140 Human
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Alternative names
- ANFB_HUMAN antibody
- BNP antibody
- BNP(1-32) antibody
see all
Images
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Western blot - Anti-proBNP antibody [C1] (ab239514)All lanes : Anti-proBNP antibody [C1] (ab239514) at 3 µg/ml
Lane 1 : Human serum
Lane 2 : Rat heart tissue lysate
Secondary
All lanes : HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution -
![Western blot - Anti-proBNP antibody [C1] (ab239514)](https://www.abcam.com/ps/products/239/ab239514/Images/ab239514-317932-anti-probnp-antibody-c1-western-blot.jpg)
Western blot - Anti-proBNP antibody [C1] (ab239514)Anti-proBNP antibody [C1] (ab239514) at 3 µg/ml + Pig heart lysate
Secondary
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab239514 has not yet been referenced specifically in any publications.