Anti-proBNP antibody [C1] (ab239514)

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Western blot - Anti-proBNP antibody [C1] (ab239514)
  • Western blot - Anti-proBNP antibody [C1] (ab239514)

Key features and details

  • Mouse monoclonal [C1] to proBNP
  • Suitable for: WB
  • Reacts with: Rat, Human, Pig
  • Isotype: IgG2a

Overview

  • Product name

    Anti-proBNP antibody [C1]
    See all proBNP primary antibodies

  • Description

    Mouse monoclonal [C1] to proBNP

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Rat, Human, Pig
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide corresponding to Mouse proBNP aa 1-100 conjugated to ovalbumin.
    Database link: P40753-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human serum. Pig and rat heart tissue lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.5 - 2 µg/ml.
Notes
WB
Use a concentration of 0.5 - 2 µg/ml.

Target

  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANFB_HUMAN antibody
      • BNP antibody
      • BNP(1-32) antibody
      • BNP(5-29) antibody
      • BNP-32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Gamma-brain natriuretic peptide antibody
      • Natriuretic peptide precursor B antibody
      • nppb antibody
      • NT proBNP antibody
      see all

    Images

    • Western blot - Anti-proBNP antibody [C1] (ab239514)
      Western blot - Anti-proBNP antibody [C1] (ab239514)
      All lanes : Anti-proBNP antibody [C1] (ab239514) at 3 µg/ml

      Lane 1 : Human serum
      Lane 2 : Rat heart tissue lysate

      Secondary
      All lanes : HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution
    • Western blot - Anti-proBNP antibody [C1] (ab239514)
      Western blot - Anti-proBNP antibody [C1] (ab239514)
      Anti-proBNP antibody [C1] (ab239514) at 3 µg/ml + Pig heart lysate

      Secondary
      HRP-Goat Anti-Mouse IgG at 0.2 µg/ml

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239514? Please let us know so that we can cite the reference in this datasheet.

    ab239514 has not yet been referenced specifically in any publications.

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