Anti-proBNP antibody [C11] (ab239519)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
  • Western blot - Anti-proBNP antibody [C11] (ab239519)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)

Key features and details

  • Mouse monoclonal [C11] to proBNP
  • Suitable for: IHC-P, WB
  • Reacts with: Human, Pig
  • Isotype: IgG2b

Overview

  • Product name

    Anti-proBNP antibody [C11]
    See all proBNP primary antibodies

  • Description

    Mouse monoclonal [C11] to proBNP

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human, Pig

  • Immunogen

    Synthetic peptide corresponding to Human proBNP aa 103-120 conjugated to ovalbumin.
    Sequence:

    SPKMVQGSGCFGRKMDRI


    Database link: P16860
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Pig skeletal muscle lysate. IHC-P: Human liver, stomach, glioma and lung cancer tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab239519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 30 µg/ml.
WB Use a concentration of 0.5 - 5 µg/ml.

Target

  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.

  • Sequence similarities

    Belongs to the natriuretic peptide family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANFB_HUMAN antibody
      • BNP antibody
      • BNP(1-32) antibody
      • BNP(5-29) antibody
      • BNP-32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Gamma-brain natriuretic peptide antibody
      • Natriuretic peptide precursor B antibody
      • nppb antibody
      • NT proBNP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)

      Formalin-fixed, paraffin-embedded human lung cancer tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.

    • Western blot - Anti-proBNP antibody [C11] (ab239519)
      Western blot - Anti-proBNP antibody [C11] (ab239519)
      Anti-proBNP antibody [C11] (ab239519) at 2 µg/ml + Pig skeletal muscle lysate

      Secondary
      HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)

      Formalin-fixed, paraffin-embedded human stomach tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)

      Formalin-fixed, paraffin-embedded human liver tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)

      Formalin-fixed, paraffin-embedded human glioma tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239519? Please let us know so that we can cite the reference in this datasheet.

    ab239519 has not yet been referenced specifically in any publications.

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