Anti-proBNP antibody [C11] (ab239519)
Key features and details
- Mouse monoclonal [C11] to proBNP
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG2b
Overview
-
Product name
Anti-proBNP antibody [C11]
See all proBNP primary antibodies -
Description
Mouse monoclonal [C11] to proBNP -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Synthetic peptide corresponding to Human proBNP aa 103-120 conjugated to ovalbumin.
Sequence:SPKMVQGSGCFGRKMDRI
Database link: P16860 -
Positive control
- WB: Pig skeletal muscle lysate. IHC-P: Human liver, stomach, glioma and lung cancer tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C11 -
Isotype
IgG2b -
Light chain type
lambda -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab239519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | Use a concentration of 5 - 30 µg/ml. | |
WB | Use a concentration of 0.5 - 5 µg/ml. |
Target
-
Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
- Unigene: 219140 Human
-
Alternative names
- ANFB_HUMAN antibody
- BNP antibody
- BNP(1-32) antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-proBNP antibody [C11] (ab239519) at 2 µg/ml + Pig skeletal muscle lysate
Secondary
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
Formalin-fixed, paraffin-embedded human stomach tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
Formalin-fixed, paraffin-embedded human liver tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-proBNP antibody [C11] (ab239519)
Formalin-fixed, paraffin-embedded human glioma tissue stained for proBNP with ab239519 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239519 has not yet been referenced specifically in any publications.