Anti-procollagen type IIA antibody [2] (ab17771)
Key features and details
- Mouse monoclonal [2] to procollagen type IIA
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-procollagen type IIA antibody [2] -
Description
Mouse monoclonal [2] to procollagen type IIA -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Epitope
Epitope is located at the amino-terminal part of procollagen type IIA. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the precursor and released into the circulation. Procollagen type II N-terminal propeptide is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 1280 Human
- Omim: 120140 Human
- SwissProt: P02458 Human
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Alternative names
- Alpha 1 type II collagen antibody
- Collagen II alpha 1 polypeptide antibody
- Procollagen type II alpha 1 antibody
Images
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ab17771, at a 1/1000 dilution, staining fetal bone anlage in the hand (around week 10) by IHC-P.
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ab17771, at a 1/1000 dilution, staining specific tumour cells of biopsy from a chondroblastoma by IHC-P. The antibody was detected with an HRP conjugated Goat anti-Mouse antibody.
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ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P. The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer. The blocking agent was 1% TSA at 25°C for 1 hour. The primary antibody was incubated (undiluted) at 25°C for 15 hours. A biotinylated anti-mouse was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab17771 has been referenced in 4 publications.
- Chen L et al. Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation. Aging (Albany NY) 13:11433-11454 (2021). PubMed: 33839696
- Qiu F et al. Development and Verification of an Immune-Based Gene Signature for Risk Stratification and Immunotherapeutic Efficacy Assessment in Gastric Cancer. Dis Markers 2021:4251763 (2021). PubMed: 34804261
- Olderøy MØ et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One 9:e91662 (2014). IHC-P ; Human . PubMed: 24626259
- Lee CH et al. Protein-releasing polymeric scaffolds induce fibrochondrocytic differentiation of endogenous cells for knee meniscus regeneration in sheep. Sci Transl Med 6:266ra171 (2014). PubMed: 25504882