Anti-PRODH antibody (ab203875)
Key features and details
- Rabbit polyclonal to PRODH
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PRODH antibody
See all PRODH primary antibodies -
Description
Rabbit polyclonal to PRODH -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PRODH aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O43272 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe please use dilutions of 1/50-1/200. |
Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe please use dilutions of 1/50-1/200. |
Target
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Function
Converts proline to delta-1-pyrroline-5-carboxylate. -
Tissue specificity
Expressed in lung, skeletal muscle and brain, to a lesser extent in heart and kidney, and weakly in liver, placenta and pancreas. -
Pathway
Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. -
Involvement in disease
Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) [MIM:239500]. HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome.
Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4) [MIM:600850]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
Sequence similarities
Belongs to the proline oxidase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 19125 Mouse
- Entrez Gene: 680409 Rat
- SwissProt: Q9WU79 Mouse
- Unigene: 28456 Mouse
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Alternative names
- HSPOX2 antibody
- mitochondrial antibody
- P53 induced gene 6 protein antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PRODH using ab203875 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse kidney tissue, labeling PRODH using ab203875 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab203875 has been referenced in 3 publications.
- Shao W et al. OIP5-AS1 specifies p53-driven POX transcription regulated by TRPC6 in glioma. J Mol Cell Biol N/A:N/A (2021). PubMed: 33508123
- Liu N et al. L-Proline Activates Mammalian Target of Rapamycin Complex 1 and Modulates Redox Environment in Porcine Trophectoderm Cells. Biomolecules 11:N/A (2021). PubMed: 34067570
- Yen CA & Curran SP Incomplete proline catabolism drives premature sperm aging. Aging Cell 20:e13308 (2021). PubMed: 33480139