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    prodh2-antibody-ab151130.pdf

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Anti-PRODH2 antibody (ab151130)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH2 antibody (ab151130)
  • Western blot - Anti-PRODH2 antibody (ab151130)
  • Immunocytochemistry - Anti-PRODH2 antibody (ab151130)

Key features and details

  • Rabbit polyclonal to PRODH2
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PRODH2 antibody
  • Description

    Rabbit polyclonal to PRODH2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to amino acids 206-286 of Human PRODH2 (UniProt: Q9UF12).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human liver tissue WB: RT4, U-25 MG, human plasma, Human liver lysate. ICC: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.
ICC
Use at an assay dependent concentration.
Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.
ICC
Use at an assay dependent concentration.

Target

  • Function

    Converts proline to delta-1-pyrroline-5-carboxylate.
  • Pathway

    Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
  • Sequence similarities

    Belongs to the proline oxidase family.
  • Target information above from: UniProt accession Q9UF12 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58510 Human
    • SwissProt: Q9UF12 Human
    • Unigene: 515366 Human
    • Alternative names

      • HsPOX1 antibody
      • Kidney and liver proline oxidase 1 antibody
      • Probable proline dehydrogenase 2 antibody
      • Probable proline oxidase 2 antibody
      • PROD2_HUMAN antibody
      • PRODH2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH2 antibody (ab151130)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH2 antibody (ab151130)
      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRODH2 with ab151130 at 1/500 dilution.
    • Western blot - Anti-PRODH2 antibody (ab151130)
      Western blot - Anti-PRODH2 antibody (ab151130)
      All lanes : Anti-PRODH2 antibody (ab151130) at 0.4 µg/ml

      Lane 1 : RT4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human plasma
      Lane 4 : Human liver tissue lysate

      Predicted band size: 59 kDa

    • Immunocytochemistry - Anti-PRODH2 antibody (ab151130)
      Immunocytochemistry - Anti-PRODH2 antibody (ab151130)

      HepG2 cells stained for PRODH2 (green) using ab151130 at 2 µg/ml dilution in ICC/IF. 

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab151130? Please let us know so that we can cite the reference in this datasheet.

    ab151130 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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