Anti-PRODH2 antibody (ab151130)
Key features and details
- Rabbit polyclonal to PRODH2
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRODH2 antibody -
Description
Rabbit polyclonal to PRODH2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to amino acids 206-286 of Human PRODH2 (UniProt: Q9UF12).
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Positive control
- IHC-P: Human liver tissue WB: RT4, U-25 MG, human plasma, Human liver lysate. ICC: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.
|
|
| ICC |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa. |
|
ICC
Use at an assay dependent concentration. |
Target
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Function
Converts proline to delta-1-pyrroline-5-carboxylate. -
Pathway
Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. -
Sequence similarities
Belongs to the proline oxidase family. - Information by UniProt
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Database links
- Entrez Gene: 58510 Human
- SwissProt: Q9UF12 Human
- Unigene: 515366 Human
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Alternative names
- HsPOX1 antibody
- Kidney and liver proline oxidase 1 antibody
- Probable proline dehydrogenase 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRODH2 antibody (ab151130)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRODH2 with ab151130 at 1/500 dilution.
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Western blot - Anti-PRODH2 antibody (ab151130)All lanes : Anti-PRODH2 antibody (ab151130) at 0.4 µg/ml
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Predicted band size: 59 kDa -
Immunocytochemistry - Anti-PRODH2 antibody (ab151130)HepG2 cells stained for PRODH2 (green) using ab151130 at 2 µg/ml dilution in ICC/IF.
Protocols
Datasheets and documents
References (0)
ab151130 has not yet been referenced specifically in any publications.
