7-AAD Staining Solution (ab228563)
Overview
-
Product name
7-AAD Staining Solution -
Sample type
Suspension cells -
Assay type
Cell-based -
Product overview
7-Amino-Actinomycin D (7-AAD) is ready-to-use nucleic acid dye solution (2 ml). 7-AAD can be used in place of propidium iodide (PI) for the exclusion of non-viable cells in flow cytometry analysis. This product can be used in combination with PE (phycoerythrin), and FITC conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is the minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Exitation = 540 nm / emission = 645 nm.
-
Tested applications
Suitable for: Flow Cytmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 400 tests 7-AAD Staining Solution 1 x 2ml
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 106 cells.
Incubate for 5 minutes at room temperature before analysis. |
| Notes |
|---|
|
Flow Cyt
Use 5µl for 106 cells. Incubate for 5 minutes at room temperature before analysis. |
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab228563 has not yet been referenced specifically in any publications.