Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 50 min
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 20 µM
Product nameAcetate Assay Kit (Colorimetric)
Sample typeSerum, Plasma, Tissue, Adherent cells, Suspension cells
Assay typeEnzyme activity (quantitative)
Sensitivity< 20 µM
Assay time0h 50m
Species reactivityReacts with: Mammals, Other species
Acetate Assay Kit (Colorimetric) (ab204719) acetate is an assay where free acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD=450 nm. This kit is rapid, simple and sensitive. It can detect less than 20 µM free acetate in various samples.
Acetate assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 40 min
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K658 Acetate Colorimetric Assay Kit. K658-100 is the same size as the 100 test size of ab204719.
Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Acetate Assay Buffer 1 x 27ml Acetate Enzyme Mix (Lyophilized) 1 vial Acetate Standard (Lyophilized) 1 vial Acetate Substrate Mix (Lyophilized) 1 vial ATP (Lyophilized) 1 vial Probe (Lyophilized) 1 vial
ab204719 has been referenced in 12 publications.
- Jin S et al. Spinal astrocyte aldehyde dehydrogenase-2 mediates ethanol metabolism and analgesia in mice. Br J Anaesth 127:296-309 (2021). PubMed: 33934892
- Li M et al. Monocarboxylate transporter 1 promotes proliferation and invasion of renal cancer cells by mediating acetate transport. Cell Biol Int 45:1278-1287 (2021). PubMed: 33559958
- Montero-Blay A et al. Inferring Active Metabolic Pathways from Proteomics and Essentiality Data. Cell Rep 31:107722 (2020). PubMed: 32492430
- Odera JO et al. NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on esophageal squamous cell carcinoma. Biochem J 477:3075-3089 (2020). PubMed: 32776152
- Lu M et al. ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition. Cell Metab 29:886-900.e5 (2019). PubMed: 30661930