Acetate Assay Kit (Colorimetric) (ab204719)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 50 min
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 20 µM
Overview
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Product name
Acetate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
< 20 µM -
Assay time
0h 50m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Acetate Assay Kit (Colorimetric) (ab204719) acetate is an assay where free acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD=450 nm. This kit is rapid, simple and sensitive. It can detect less than 20 µM free acetate in various samples.
Acetate assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 40 min
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K658 Acetate Colorimetric Assay Kit. K658-100 is the same size as the 100 test size of ab204719.
Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Acetate Assay Buffer 1 x 27ml Acetate Enzyme Mix (Lyophilized) 1 vial Acetate Standard (Lyophilized) 1 vial Acetate Substrate Mix (Lyophilized) 1 vial ATP (Lyophilized) 1 vial Probe (Lyophilized) 1 vial
Images
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Acetate Assay Kit (Colorimetric) (ab204719)
Acetate Standard Curve.
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Acetate Assay Kit (Colorimetric) (ab204719)Measurement of Acetate in human serum (5 μl), fish liver (~100 μg) and egg (~200 μg).
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab204719 has been referenced in 12 publications.
- Jin S et al. Spinal astrocyte aldehyde dehydrogenase-2 mediates ethanol metabolism and analgesia in mice. Br J Anaesth 127:296-309 (2021). PubMed: 33934892
- Li M et al. Monocarboxylate transporter 1 promotes proliferation and invasion of renal cancer cells by mediating acetate transport. Cell Biol Int 45:1278-1287 (2021). PubMed: 33559958
- Montero-Blay A et al. Inferring Active Metabolic Pathways from Proteomics and Essentiality Data. Cell Rep 31:107722 (2020). PubMed: 32492430
- Odera JO et al. NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on esophageal squamous cell carcinoma. Biochem J 477:3075-3089 (2020). PubMed: 32776152
- Lu M et al. ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition. Cell Metab 29:886-900.e5 (2019). PubMed: 30661930