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Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)

Reviews (2)Q&A (5)References (46)
Evaluation of acetylcholinesterase (AChE) activity using ab138871
  • Functional Studies - Acetylcholinesterase Assay Kit (ab138871)
  • Functional Studies - Acetylcholinesterase Assay Kit (ab138871)
  • Functional Studies - Acetylcholinesterase Assay Kit (ab138871)
  • Sample Standard Curve for Acetylcholinesterase

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 30 min
  • Sample type: Cell culture extracts, Cell Lysate, Plasma, Serum, Tissue Lysate
  • Sensitivity: 1 mU/ml

Overview

  • Product name

    Acetylcholinesterase Assay Kit (Colorimetric)
    See all Acetylcholinesterase kits

  • Detection method

    Colorimetric

  • Sample type

    Serum, Plasma, Cell culture extracts, Cell Lysate, Tissue Lysate

  • Assay type

    Enzyme activity

  • Sensitivity

    1 mU/ml

  • Assay time

    0h 30m

  • Product overview

    Acetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.


    The acetylcholinesterase assay protocol uses DTNB to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The absorption intensity of DTNB adduct (410 nm) is used to measure the amount of thiocholine formed, which is proportional to the AChE activity.


    The assay will detect as little as 0.1 mU AChE in a 100 µL assay volume (1 mU/mL).  


    Acetylcholinesterase assay protocol summary:
    - add standards and samples to wells
    - add acetylthiocholine reaction mix and incubate for 10-30 min at room temp
    - analyze with a microplate reader

  • Notes

    Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.

    If you are looking for a fluorometric kit, we recommend Acetylcholinesterase Assay Kit (Fluorometric-Green) (ab133872) (Ex/Em = 490/520 nm) or Acetylcholinesterase Assay Kit (Fluorometric-Red) (ab133873) (Ex/Em = 540/590 nm).

  • Platform

    Microplate reader

Properties

Images

  • Evaluation of acetylcholinesterase (AChE) activity using ab138871
    Evaluation of acetylcholinesterase (AChE) activity using ab138871Morzelle M et al., PLoS One, 11(11). Fig 6. doi: 10.1371/journal.pone.0166123 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ab138871 was used to determine AChE activity in the cortex and in the hippocampus. AChE activity was measured at 410 nm.

  • Functional Studies - Acetylcholinesterase Assay Kit (ab138871)
    Functional Studies - Acetylcholinesterase Assay Kit (ab138871)

    AChE measured in mouse tissue lysates showing quantity (mU) per mg protein of tested sample

  • Functional Studies - Acetylcholinesterase Assay Kit (ab138871)
    Functional Studies - Acetylcholinesterase Assay Kit (ab138871)

    AChE measured in cell lysates showing quantity (mU) per 1 mln of tested cells

  • Functional Studies - Acetylcholinesterase Assay Kit (ab138871)
    Functional Studies - Acetylcholinesterase Assay Kit (ab138871)

    AChE measured in biological fluids showing quantity (mU) per mL of tested sample

  • Sample Standard Curve for Acetylcholinesterase
    Sample Standard Curve for Acetylcholinesterase
    Acetylcholinesterase dose response was measured in a white/clear bottom 96-well plate with ab138871 Acetylcholinesterase Assay Kit (Colorimetric) using a microplate reader. As low as 0.1 mU/well of acetylcholinesterase can be detected with 30 minutes incubation (n=3)

References (46)

Publishing research using ab138871? Please let us know so that we can cite the reference in this datasheet.

ab138871 has been referenced in 46 publications.

  • Zhang H  et al. The Effect of Guilingji Capsules on Vascular Mild Cognitive Impairment: A Randomized, Double-Blind, Controlled Trial. Evid Based Complement Alternat Med 2022:4778163 (2022). PubMed: 35116067
  • Aykac A  et al. A multi-parameter evaluation of the neuroprotective and cognitive-enhancing effects of Origanum onites L. (Turkish Oregano) essential oil on scopolamine-induced amnestic rats. Metab Brain Dis 37:1041-1055 (2022). PubMed: 35201555
  • Girard MP  et al. Chemical Synthesis and Biological Activities of Amaryllidaceae Alkaloid Norbelladine Derivatives and Precursors. Molecules 27:N/A (2022). PubMed: 36080382
  • Guo Q  et al. Novel small molecular compound 2JY-OBZ4 alleviates AD pathology in cell models via regulating multiple targets. Aging (Albany NY) 14:8077-8094 (2022). PubMed: 36227154
  • Uras G  et al. Development of p-Tau Differentiated Cell Model of Alzheimer's Disease to Screen Novel Acetylcholinesterase Inhibitors. Int J Mol Sci 23:N/A (2022). PubMed: 36499118
View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Zebrafish Samples

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
We have tested this product with zebrafish brain and embryo lysates. A zebrafish mutant line sb55 (Behra et al., 2002) was used for the validation experiments as a control. In this mutant line homozygous mutants sb55-/- has abolished acetycholinesterase activity compared to wildype controls and homozygous mutation is lethal for embryos after 4 dpf due to paralysis. The measurement taken from homozygous mutants at 4dpf is approximately zero and there is a drastic difference between mutants and wildtypes at 4dpf in terms of acetylcholinesterase activity. In the adult fish mutation is not lethal for heterozygous mutants(sb55+/-) and heterozygous mutants have lower levels of brain acetylcholinesterase (Ninkovic et al., 2006). Our data is also confirming the previous observations by other groups working with zebrafish model and indicate that this product is giving quite accurate measurements with zebrafish brain and embryo samples.

Extraction Protocol: Brain tissues and embryos were homogenized in ice cold 100 ul of 25 mM Tris HCl pH:8. Then centrifuged at 13 000 rpm for 20 mins at 4°C. Collected supernatants were used for the assay.


Behra, M., Cousin, X., Bertrand, C., Vonesch, J.L., Biellmann, D., Chatonnet, A., and Strähle, U. (2002) Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo. Nature Neuroscience. 5(2):111-118.

Ninkovic, J., Folchert, A., Makhankov, Y. V., Neuhauss, S. C., Sillaber, I., Straehle, U., & Bally‐Cuif, L. (2006). Genetic identification of AChE as a positive modulator of addiction to the psychostimulant D‐amphetamine in zebrafish. Journal of neurobiology, 66(5), 463-475.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Elif Karoğlu

Verified customer

Submitted May 10 2019

ab138871 can be used for Exosomes quantification in cell supernatant

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
The kit is very easy to use, precise and fast.
I wanted to determine Exosomes presence and amount in the supernatant of cells and I was very satisfied with the result obtained with the kit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 04 2017

Question

Do you have any suggestion about which would be the most appropriate lysis buffer in order to keep the enzyme in its active form?

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Answer

Our Mammalian Cell Lysis Buffer 5X (ab179835) would be the best lysis buffer to use.

<1%Chaps also is OK. We have not checked NP40 and RIPA, but they should be OK theoretically.

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Question

Preparation of lysate of lung cancer cells

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Answer

Our recommended buffer for preparation of cell or tissue lysates for the Acetylcholinesterase assay ab138871 is ab179835.

https://www.abcam.com/Mammalian-Cell-Lysis-Buffer-5X-ab179835.html

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Question

Will ethanol inhibit the functionality of the assay?

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Answer

We didn't test the inhibitory effect of ethanol on acetylcholinesterase, but the publications below showed that ethanol will inhibit activity of AChE.

http://www.ncbi.nlm.nih.gov/pubmed/3767274
http://link.springer.com/article/10.1023%2FA%3A1007555924661

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Question

I have purchased the acetylcholinesterase assay kit (ab138871) and would like to assess activity in rat blood. Could you please advise on what is the best method to do this. Also do you know if anyone has tried to measure in rat blood.

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Answer



The recommended protocol to prepare whole blood for measuring Acetylcholinesterase is as follows:

Whole blood and red blood cells (RBC) lysates can be prepared by diluting 1.0 ml of blood or RBC in 19.0 ml of 0.03% Triton X-100 in water. And then diluted in dilutions in Assay Buffer for further experiment.

All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Therefore, unfortunately, there aren’t any references from other customers, at least that we know, using this kit with rat blood.

However, please bear in mind that the Abpromise® guarantee ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 12 months of purchase.

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Question

Llamada de telefono. Cuantos ensayos pueden hacerse con un kit?

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Answer

Gracias por contactarnos.
Me confirman mis compañeros del laboratorio que el kit es válido para realizar unos 200 ensayos, teniendo en cuenta un volumen aproximado de muestra por ensayo de 50uL.
Espero que esta información sea útil. En caso contrario, no dudes en contactarnos de nuevo.

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