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Acetylcholinesterase Assay Kit (Fluorometric - Green) (ab138872)

Submit a review Q&A (1)References (2)
Sample Standard Curve for Acetylcholinesterase

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Cell culture extracts, Whole Blood
    • Sensitivity: 0.1 mU/ml

    Overview

    • Product name

      Acetylcholinesterase Assay Kit (Fluorometric - Green)
      See all Acetylcholinesterase kits

    • Detection method

      Fluorescent

    • Sample type

      Cell culture extracts, Whole Blood

    • Assay type

      Enzyme activity

    • Sensitivity

      0.1 mU/ml

    • Species reactivity

      Reacts with: Mammals, Other species

    • Product overview

      Acetylcholinesterase Assay KIt (Fluorometric - Green) (ab138872) uses an outstanding Thiol Green Indicator to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions. Thiol Green Indicator is not fluorescent until reacted with a thiol group. It has spectral properties similar to those of fluorescein, making this assay compatible with almost every fluorescence instrument. The fluorescence intensity of Thiol Green Indicator is used to measure AChE activity. Compared to the existing thiol probes (e.g., mBBr and bBBr), Thiol Green Indicator is much more sensitive.


      This product is an optimized "mix-and-read" assay that provides an ultrasensitive fluorometric one-step protocol to detect as little as 0.01mU AChE in a 100 µl assay volume (0.1 mU/ml). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. 


       


      Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.


       


      If you are looking for a colorimetric kit, we recommend Acetylcholinesterase Assay Kit (Colorimetric) (ab138871). If your plate reader has a filter to measure red fluorescnce (Ex/Em = 540/590 nm), you can use Acetylcholinesterase Assay Kit (Fluorometric - Red) (ab138873).

    • Notes

      Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer’s disease (AD) and myasthenia gravis.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 200 tests
      Acetylcholinesterase Standard 1 vial
      Acetylthiocholine 1 vial
      Assay Buffer 1 x 25ml
      DMSO 1 x 100µl
      Thiol Green Indicator 1 vial

    • Research areas

    • Function

      Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

    • Tissue specificity

      Isoform H is highly expressed in erythrocytes.

    • Sequence similarities

      Belongs to the type-B carboxylesterase/lipase family.

    • Cellular localization

      Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
    • Information by UniProt

    • Alternative names

      • ACEE
      • ACES_HUMAN
      • Acetylcholinesterase
      • AChE
      • Apoptosis related acetylcholinesterase
      • ARACHE
      • N ACHE
      • N-ACHE
      • YT
      • YT blood group
      see all

    Images

    • Sample Standard Curve for Acetylcholinesterase
      Sample Standard Curve for Acetylcholinesterase
      Acetylcholinesterase dose response was measured in a solid black 96-well plate with ab138872 using a fluorescence microplate reader. As low as 0.01 mU/well of acetylcholinesterase can be detected with 20 minutes incubation (n=3).

    References (2)

    Publishing research using ab138872? Please let us know so that we can cite the reference in this datasheet.

    ab138872 has been referenced in 2 publications.

    • Shim KH  et al. Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson's Disease. Front Aging Neurosci 13:665400 (2021). PubMed: 34122043
    • Transfiguracion J  et al. Rapid In-Process Monitoring of Lentiviral Vector Particles by High-Performance Liquid Chromatography. Mol Ther Methods Clin Dev 18:803-810 (2020). PubMed: 32953931

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I have run out of Assay Buffer but the other reagents have remaining volume. Can I get the recipe of the Assay Buffer to make some in house?

    Read More
    Answer

    You can use 50 mM Na phosphate buffer, pH 6.5.

    Read More

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