Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Whole Blood
- Sensitivity: 0.1 mU/ml
Product nameAcetylcholinesterase Assay Kit (Fluorometric - Green)
See all Acetylcholinesterase kits
Sample typeCell culture extracts, Whole Blood
Assay typeEnzyme activity
Species reactivityReacts with: Mammals, Other species
Acetylcholinesterase Assay KIt (Fluorometric - Green) (ab138872) uses an outstanding Thiol Green Indicator to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions. Thiol Green Indicator is not fluorescent until reacted with a thiol group. It has spectral properties similar to those of fluorescein, making this assay compatible with almost every fluorescence instrument. The fluorescence intensity of Thiol Green Indicator is used to measure AChE activity. Compared to the existing thiol probes (e.g., mBBr and bBBr), Thiol Green Indicator is much more sensitive.
This product is an optimized "mix-and-read" assay that provides an ultrasensitive fluorometric one-step protocol to detect as little as 0.01mU AChE in a 100 µl assay volume (0.1 mU/ml). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.
Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.
If you are looking for a colorimetric kit, we recommend Acetylcholinesterase Assay Kit (Colorimetric) (ab138871). If your plate reader has a filter to measure red fluorescnce (Ex/Em = 540/590 nm), you can use Acetylcholinesterase Assay Kit (Fluorometric - Red) (ab138873).
Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer’s disease (AD) and myasthenia gravis.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Acetylcholinesterase Standard 1 vial Acetylthiocholine 1 vial Assay Buffer 1 x 25ml DMSO 1 x 100µl Thiol Green Indicator 1 vial
FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Tissue specificityIsoform H is highly expressed in erythrocytes.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
- Information by UniProt
ab138872 has been referenced in 2 publications.
- Shim KH et al. Decreased Exosomal Acetylcholinesterase Activity in the Plasma of Patients With Parkinson's Disease. Front Aging Neurosci 13:665400 (2021). PubMed: 34122043
- Transfiguracion J et al. Rapid In-Process Monitoring of Lentiviral Vector Particles by High-Performance Liquid Chromatography. Mol Ther Methods Clin Dev 18:803-810 (2020). PubMed: 32953931