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Signal Transduction Metabolism Amino Acids
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Adenosylhomocysteinase (AHCY) Inhibitor Assay Kit (Fluorometric) (ab204694)

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Adenosylhomocysteinase (AHCY) Inhibitor Screening Kit (Fluorometric) (ab204694)

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Inhibitor compounds

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    Overview

    • Product name

      Adenosylhomocysteinase (AHCY) Inhibitor Assay Kit (Fluorometric)
      See all SAHH kits
    • Detection method

      Fluorescent
    • Sample type

      Inhibitor compounds
    • Assay type

      Enzyme activity
    • Product overview

      Adenosylhomocysteinase (AHCY) Inhibitor Screning Kit (Fluorometric) (ab204694) is a simple assay to study, screen or characterize potential inhibitors of AHCY. In this assay, the homocysteine generated from the breakdown of S-Adenosyl Homocysteine (SAH) is measured using a Thiol Detecting Reagent which results in enhanced fluorescence that can be measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible, and therefore Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K326 AHCY Inhibitor Screening Kit (Fluorometric). K326-100 is the same size as the 100 test size of ab204694.

      Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies).

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      3-Deazaneplanocin A (10 μM) (in DMSO) 1 x 10µl
      Adenosine Deaminase 1 vial
      AHCY Assay Buffer 1 x 25ml
      AHCY Enzyme 1 vial
      AHCY Reconstitution Buffer 1 x 250µl
      AHCY Substrate (in DMSO) 1 x 100µl
      Thiol Detecting Reagent (in DMSO) 1 x 200µl
    • Research areas

      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
    • Function

      Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
    • Pathway

      Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
    • Involvement in disease

      Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
    • Sequence similarities

      Belongs to the adenosylhomocysteinase family.
    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P23526 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Adenosylhomocysteinase
      • AdoHcyase
      • ahcY
      • S adenosyl L homocysteine hydrolase
      • S adenosylhomocysteine hydrolase
      • S-adenosyl-L-homocysteine hydrolase
      • SAHH
      • SAHH_HUMAN
      see all

    Associated products

    • Related Products

      • Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric) (ab197002)

    Images

    • Adenosylhomocysteinase (AHCY) Inhibitor Screening Kit (Fluorometric) (ab204694)
      Adenosylhomocysteinase (AHCY) Inhibitor Screening Kit (Fluorometric) (ab204694)

      Inhibition of Adenosylhomocysteinase (AHCY) Enzyme activity by 3-Deazaneplanocin A. IC50 of 3-Deazaneplanocin A was determined to be 0.137 nM. 

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab204694? Please let us know so that we can cite the reference in this datasheet.

    ab204694 has been referenced in 1 publication.

    • Fukumoto K  et al. Excess S-adenosylmethionine inhibits methylation via catabolism to adenine. Commun Biol 5:313 (2022). PubMed: 35383287

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