ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 µM
Product nameADP Assay Kit (Colorimetric/Fluorometric)
See all ADP kits
Sample typeUrine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
Sensitivity> 1 µM
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides a convenient colorimetric and fluorometric method to measure ADP level.
In the ADP assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (ODmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm).
Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. In comparison, this assay is simple, sensitive, stable and high-throughput adaptable and can be used with conventional microplate readers.
The ADP assay can detect as low as 1 μM ADP in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K355 ADP Colorimetric/Fluorometric Assay Kit. K355-100 is the same size as the 100 test size of ab83359.
ADP is a product of ATP de-phosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests ADP Assay Buffer WM 1 x 25ml ADP Converter (lyophilized) Purple 1 vial ADP Developer Mix (lyophilized) Green 1 vial ADP Probe (in DMSO) Red 1 x 200µl ADP Standard (1 µmole) (lyophilized) Yellow 1 vial
RelevanceADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
- Adenosine diphosphate
ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).
ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.
Colorimetric and Fluorometric examples of ADP Standard curve.
Datasheets and documents
ab83359 has been referenced in 24 publications.
- Rajagopalan KS et al. Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells. Cells 11:N/A (2022). PubMed: 35681498
- Markussen LK et al. Lipolysis regulates major transcriptional programs in brown adipocytes. Nat Commun 13:3956 (2022). PubMed: 35803907
- Liu K et al. Identification of super-enhancer-associated transcription factors regulating glucose metabolism in poorly differentiated thyroid carcinoma. Genet Mol Biol 45:e20210370 (2022). PubMed: 36121916
- Almeida L et al. Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis. Immunity 54:68-83.e6 (2021). PubMed: 33238133
- Farahani RA et al. Renal ischemia alters expression of mitochondria-related genes and impairs mitochondrial structure and function in swine scattered tubular-like cells. Am J Physiol Renal Physiol 319:F19-F28 (2020). PubMed: 32463728