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ALDH Activity Assay Kit (Colorimetric) (ab155893)

Reviews (1)Q&A (3)References (11)
ALDH Activity Assay kit (ab155893)
  • ALDH activity in Positive Control
  • ALDH Activity Assay kit (ab155893)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 30 min
  • Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Culture Media
  • Sensitivity: 0.5 mU/well

Overview

  • Product name

    ALDH Activity Assay Kit (Colorimetric)

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Culture Media

  • Assay type

    Enzyme activity (quantitative)

  • Sensitivity

    < 0.5 mU/well

  • Assay time

    0h 30m

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity in a variety of samples.


    ALDH assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 20-60 min
    - analyze with a microplate reader at 5 min and 20-60 min

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K731 Aldehyde Dehydrogenase Activity Colorimetric Assay Kit. K731-100 is the same size as the 100 test size of ab155893.

    The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Acetaldehyde Purple 1 x 0.5ml
    ALDH Assay Buffer WM 1 x 25ml
    ALDH Positive Control Green 1 vial
    ALDH Substrate Mix Red 1 vial
    NADH Standard Yellow 1 vial

  • Research areas

Images

  • ALDH Activity Assay kit (ab155893)
    ALDH Activity Assay kit (ab155893)

    Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.

  • ALDH activity in Positive Control
    ALDH activity in Positive Control

    Typical OD of the diffrent positive contols mesuared over 1 hour. 

  • ALDH Activity Assay kit (ab155893)
    ALDH Activity Assay kit (ab155893)

    ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.

References (11)

Publishing research using ab155893? Please let us know so that we can cite the reference in this datasheet.

ab155893 has been referenced in 11 publications.

  • Lee HY  et al. Turmeric extract (Curcuma longa L.) regulates hepatic toxicity in a single ethanol binge rat model. Heliyon 8:e10737 (2022). PubMed: 36193527
  • Abdelwahab EMM  et al. Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations. Front Oncol 11:644592 (2021). PubMed: 34178631
  • Liu Y  et al. In vivo selection of highly metastatic human ovarian cancer sublines reveals role for AMIGO2 in intra-peritoneal metastatic regulation. Cancer Lett 503:163-173 (2021). PubMed: 33524500
  • Yano S  et al. Electrolyzed Hydrogen Water Protects against Ethanol-Induced Cytotoxicity by Regulating Aldehyde Metabolism-Associated Enzymes in the Hepatic Cell Line HepG2. Antioxidants (Basel) 10:N/A (2021). PubMed: 34069398
  • Je J  et al. Combined Water Extracts from Oxidation-Treated Leaves and Branches of Hovenia dulcis Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice. Nutrients 13:N/A (2021). PubMed: 34959956
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Measurement of ALDH activity in cancer cells

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Detailed protocol was easy followed as per kit manual for measuring the ALDH activity in lung cancer cell lines. The data is consistent with the expression level of ALDH protein in the respective cancer cell lines.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 21 2018

Question

Please inform me if the following colorimetric kit has been reported to be used for estimation of ALDh activity in cancer cell lines such as MDA-MB-231 and MCF-7



ab155893

ALDH Activity Assay Kit

(Colorimetric)





Read More
Answer

The ALDH Activity Assay Kit (Colorimetric) (ab155893) has not been tested yet in cancer cell lines such as MDA-MB-231 and MCF-7.
Unfortunately, we do not currently have more information about this product. We will update the on-line datasheet for this product as soon as we receive additional data.

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Question

I recently purchased the ALDH Activity Assay Kit (ab155893). The “positive control” is not further defined, but I assume that it consists of aldehyde dehydrogenase enzyme(s). Since there are 19 alcohol dehydrogenases in humans, it would be useful to know which one(s) is /are included in the kit.
Furthermore, I was wondering what the ALDH Substrate Mix contains, since acetaldehyde is included in a separate vial.
Kind regards,

Read More
Answer

The contents of the substrate mix is proprietary information and cannot be disclosed.
The positive control is an ALDH isolated from Baker’s yeast.

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Question

I was wondering if your ALDH activity kit is compatible with cell lysates prepared using RIPA buffer or other similar lysis buffers? If not, does your Assay Buffer serve as a lysis buffer or does the sample need to be mechanically homogenized? Also, can the lysates made in your Assay Buffer be used in other protein assays, such as Western blot or ELISA?

Read More
Answer

We would recommend using our assay buffer for the lysis, as RIPA may not contain all the components required for the reaction to occur. The samples should be homogenized in ice cold assay buffer for 10 minutes on ice (page 8 of the online protocol). Lysates prepared in the assay buffer can also be used in Western blot and ELISA.

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