Angiotensin Converting Enzyme 1 (ACE1) Inhibitor Screening Kit (Colorimetric) (ab283407)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
Overview
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Product name
Angiotensin Converting Enzyme 1 (ACE1) Inhibitor Screening Kit (Colorimetric)
See all Angiotensin Converting Enzyme 1 kits -
Detection method
Colorimetric -
Sample type
Inhibitor compounds -
Assay duration
Multiple steps standard assay -
Product overview
ACE1 Inhibitor Screening Kit (Colorimetric) (ab283407) can be used to screen/characterize potential inhibitors of ACE1.
The kit utilizes the ability of an active ACE1 to hydrolyze a synthetic substrate, which results in the decrease in OD at 345 nm. In the presence of Captopril, an ACE1 specific inhibitor, the ACE1 enzymatic activity is greatly reduced and there is no significant decrease in the OD at 345 nm.
The assay kit provides a rapid, simple and reliable test for high-throughput screening of ACE1 inhibitors.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K719 ACE1 Inhibitor Screening Kit (Colorimetric). K719-100 is the same size as the 100 test size of ab283407.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 96-Well UV Transparent Plate 1 unit Angiotensin Converting Enzyme 1 Assay Buffer 1 x 25ml Angiotensin Converting Enzyme 1 Enzyme 1 x 200µl Angiotensin Converting Enzyme 1 Inhibitor Control 1 x 100µl Angiotensin Converting Enzyme 1 Substrate 1 vial -
Research areas
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Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. -
Tissue specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. -
Involvement in disease
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ACE
- ACE 1
- ACE T
see all
Images
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Functional Studies - Angiotensin Converting Enzyme 1 (ACE1) Inhibitor Screening Kit (Colorimetric) (ab283407)
Inhibition of ACE1 activity by Captopril. IC50 was calculated to be 6.61 nM.
Datasheets and documents
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Datasheet download
References (0)
ab283407 has not yet been referenced specifically in any publications.
