Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
Product nameAngiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit
See all ACE2 kits
Assay typeEnzyme activity (quantitative)
Assay durationMultiple steps standard assay
Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit (ab273373) can be used to screen for potent inhibitors of ACE2 activity, it utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an ACE2 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE2 inhibitors in a high-throughput format.
This product is manufactured by BioVision, an Abcam company and was previously called K310 Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit. K310-100 is the same size as the 100 test size of ab273373.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests ACE2 Assay Buffer 1 x 25ml ACE2 Dilution Buffer 1 x 1.5ml ACE2 Enzyme 1 x 20µl ACE2 Inhibitor (0.5 mM) 1 x 5µl ACE2 Substrate 1 x 200µl
FunctionCarboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Tissue specificityExpressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
Sequence similaritiesBelongs to the peptidase M2 family.
modificationsN-glycosylation on Asn-90 may limit SARS infectivity.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- ACE 2
- ACE related carboxypeptidase
- ACE-related carboxypeptidase
ab273373 has not yet been referenced specifically in any publications.