Annexin V-PE Apoptosis Staining / Detection Kit (ab14155)
Key features and details
- Assay type: Direct
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 10 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Annexin V-PE Apoptosis Staining / Detection Kit
See all Annexin V/ANXA5 kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
0h 10m -
Product overview
Annexin V-PE Apoptosis Staining / Detection Kit ab14155 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
The Annexin V-PE reagent contained in the kit is also available as Annexin V-PE reagent ab14154.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K128 Annexin V-PE Apoptosis Detection Kit. K128-100 is the same size as the 100 test size of ab14155.
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Annexin V-PE 1 x 500µl Binding Buffer 1 x 50ml -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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- Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
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- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
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Images
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Functional assays: Annexin V-PE Apoptosis Detection Kit (ab14155)
Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab14155 has been referenced in 15 publications.
- Liu BH et al. Tumor Suppressive Role of MUC6 in Wilms Tumor via Autophagy-Dependent ß-Catenin Degradation. Front Oncol 12:756117 (2022). PubMed: 35574418
- Caire R et al. YAP/TAZ: Key Players for Rheumatoid Arthritis Severity by Driving Fibroblast Like Synoviocytes Phenotype and Fibro-Inflammatory Response. Front Immunol 12:791907 (2021). PubMed: 34956224
- Guo H et al. NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer. Mol Cell 81:4041-4058.e15 (2021). PubMed: 34624217
- Brescia M et al. High-Capacity Adenoviral Vectors Permit Robust and Versatile Testing of DMD Gene Repair Tools and Strategies in Human Cells. Cells 9:N/A (2020). PubMed: 32252479
- Xiang J et al. Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries. Transl Psychiatry 10:181 (2020). PubMed: 32513932