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    products/assay-kits/apoptosis-necrosis-assay-kit-blue-green-red-ab176749.pdf

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Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (6)Q&A (1)References (63)

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Jurkat cells analyzed with Apoptosis/Necrosis Detection Kit (ab176749)
  • Jurkat cells analyzed with Apoptosis/Necrosis Detection Kit (ab176749)
  • Flow cytometric analysis of Jurkat cells

Key features and details

  • Assay type: Cell-based
  • Platform: Flow cytometer, Fluorescence microscope
  • Assay time: 1 hr
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Apoptosis/ Necrosis Assay Kit (blue, green, red)
    See all Apoptosis/ Necrosis kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    1h 00m
  • Product overview

    Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.


    The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.


    Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.


    Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.


    In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm.


    This kit is optimized to simultaneously detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.


    This product was previously called Apoptosis/ Necrosis Detection Kit.


     

  • Notes

    For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    200X 7-AAD 1 x 100µl
    Apopxin Green Indicator 1 x 200µl
    Assay Buffer 1 x 50ml
    CytoCalcein Violet 450 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Apoptosis Assay Kits
    • Cell viability

Images

  • Jurkat cells analyzed with Apoptosis/Necrosis Detection Kit (ab176749)
    Jurkat cells analyzed with Apoptosis/Necrosis Detection Kit (ab176749)

    Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.

  • Jurkat cells analyzed with Apoptosis/Necrosis Detection Kit (ab176749)
    Jurkat cells analyzed with Apoptosis/Necrosis Detection Kit (ab176749)

    Fluorescent analysis of live non-induced Jurkat cells stained by CytoCalcein Violet 450. 

  • Flow cytometric analysis of Jurkat cells
    Flow cytometric analysis of Jurkat cells

    Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37 oC, 5% CO2 incubator for 5 hours, and then
    loaded with Apopxin Green Indicator for 30 minutes. The fluorescence intensity of Apopxin Green Indicator was measured with a
    flow cytometer using FL1 channel.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (63)

Publishing research using ab176749? Please let us know so that we can cite the reference in this datasheet.

ab176749 has been referenced in 63 publications.

  • Zerfaoui M  et al. Nuclear Localization of BRAFV600E Is Associated with HMOX-1 Upregulation and Aggressive Behavior of Melanoma Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35053476
  • Mashinchian O  et al. In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging. Elife 11:N/A (2022). PubMed: 35245177
  • Huang P  et al. Lysosomal ATP Transporter SLC17A9 Controls Cell Viability via Regulating Cathepsin D. Cells 11:N/A (2022). PubMed: 35269509
  • Bai H  et al. Mechanical control of innate immune responses against viral infection revealed in a human lung alveolus chip. Nat Commun 13:1928 (2022). PubMed: 35396513
  • Mishra A  et al. Human Macrophages Exhibit GM-CSF Dependent Restriction of Mycobacterium tuberculosis Infection via Regulating Their Self-Survival, Differentiation and Metabolism. Front Immunol 13:859116 (2022). PubMed: 35634283
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-7 of 7 Abreviews or Q&A

Very good kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
It’s a great tool for getting cell apoptosis info. Very easy to use and result oriented.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 02 2023

ab176749 - Apoptosis/Necrosis Detection in PC3 prostate cancer cells

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Apoptosis has been detected by AnnexinV (green fluorescence Ex/Em = 490/525 nm) an phosphatidylserine indicator on the cell surface using the Apoptosis/Necrosis Detection Kit (blue, green, red) (ab176749) (Abcam, UK) and Cell-IQ MLF live imaging system. The kit has been used according to the manufacturer’s instructions, omitting the blue dye of live cells, due to the lack of the appropriate excitation light source. Phase contrast and Green fluorescence images , monitoring apoptosis, have been merged, indicating an apoptosis increase following radiation treatment , as illustrated in the attached image.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Jun 27 2022

Apoptosis/ necrosis abtrial for Fluorescent plate reader

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I tried this kit with Fluorescent plate reader and it worked very well. Used PC12 cells for the study. Highly recommended. easy to use
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sheelu Monga

Verified customer

Submitted May 13 2021

ab176749 Apoptosis/Necrosis Detection Kit (blue, green, red) in flow cytometry

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Ab176749 has been used to detect Apoptosis/Necrosis in untreated and treated PC3 prostate cancer cells. The staining has been performed according to the manufacturer's instructions and apoptosis was clearly detected in the treated cells, as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Dec 10 2018

Apoptosis/ Necrosis Detection in proteasome inhibitor treated MEFs

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Fluorescent analysis demonstrating cells that are live (blue), apoptotic (green), and necrotic (red) in MEFs cells induced by 10 nM proteasome inhibitor for 6 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 23 2018

ab176749 - Apoptosis/Necrosis Detection in PC3 prostate cancer cells

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Apoptosis has been detected by AnnexinV (green fluorescence Ex/Em = 490/525 nm) an phosphatidylserine indicator on the cell surface using the Apoptosis/Necrosis Detection Kit (blue, green, red) (ab176749) (Abcam, UK) and Cell-IQ MLF live imaging system. The kit has been used according to the manufacturer’s instructions, omitting the blue dye of live cells, due to the lack of the appropriate excitation light source. Phase contrast and Green fluorescence images , monitoring apoptosis, have been merged, indicating an apoptosis increase following radiation treatment , as illustrated in the attached image.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Jan 17 2017

Question

How long is the kit good for? expiration date before and after use? In order to avoid multiple freeze thaws should I aliquot the dye into smaller volumes? The cytocalcien violet need to be diluted in DMSO prior to use - how long will that dye be good for after DMSO addition?

Read More

Abcam community

Verified customer

Asked on Dec 07 2015

Answer

How long is the kit good for? Expiration date before and after use?
We guarantee our products to work for 12 months after your purchase. This applies before use for ab176749 due to the expiration of the cytocalcein violet after DMSO dilution (see below).

In order to avoid multiple freeze thaws should I aliquot the dye into smaller volumes?
Yes, please make single-use aliquots and freeze.

The cytocalcein violet need to be diluted in DMSO prior to use - how long will that dye be good for after DMSO addition?
Up to 3 months.

Read More

Kevin Hanson

Abcam Scientific Support

Answered on Dec 07 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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