Apoptotic DNA Ladder Isolation Kit (ab65627)
Key features and details
- Assay type: Direct
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Apoptotic DNA Ladder Isolation Kit
See all DNA fragmentation kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Direct -
Product overview
Apoptotic DNA Ladder Isolation Kit (ab65627) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells and tissues. The new procedure selectively extracts DNA ladders without interference of intact genomic DNA in only 2-4 hours. As little as 5% of apoptotic cells in a sample can be visualized with this high sensitivity assay.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K170 Apoptotic DNA Ladder Isolation Kit. K170-50 is the same size as the 50 test size of ab65627.
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 50 tests Ammonium Acetate Solution Yellow 1 x 250µl DNA Ladder Extraction Buffer WM 1 x 12.5ml DNA Suspension Buffer Green 1 x 2ml Enzyme A Solution Blue 1 x 250µl Enzyme B (Lyophilized) Red 1 vial -
Research areas
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Relevance
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Associated products
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Images
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Functional Assay: ab65627 Apoptotic DNA Ladder Isolation Kit
Apoptosis induced in Jurkat cells (1e6 cell x mL-1) by incubation for 24 hours with 1) Control medium; 2) 2 uM Camptothecin (ab120115); 3) 10 uM Camptothecin; 4) 10 uM Camptothecin + 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487); 5) 50 ng x mL-1 anti-Fas Ab (MBL), 6) 0.05 mM H2O2 (Sigma); 7) 1 mM H2O2. 5 uL of DNA preparation loaded into each well.
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Functional Studies - Apoptotic DNA Ladder Isolation Kit (ab65627)DNA ladders were extracted from 2 x 106 Jurkat cells were treated with 2 μM Camptothecin. The kit extracts laddered DNA only but not intact genomic DNA; therefore, more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab65627 has been referenced in 6 publications.
- Matsumoto S et al. Overexpressed exocyst complex component 3-like 1 spontaneously induces apoptosis. Biomed Res 42:109-113 (2021). PubMed: 34092752
- Okada J et al. TBC1D8B, a GTPase-activating protein, is a novel apoptosis inducer. Biomed Res 42:95-102 (2021). PubMed: 34092754
- Okada J et al. FAM83G Is a Novel Inducer of Apoptosis. Molecules 25:N/A (2020). PubMed: 32570757
- Aksoy E & Azkur AK Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner. J Vet Med Sci 81:204-212 (2019). PubMed: 30541984
- Yan MD et al. The anti-tumor activity of brown seaweed oligo-fucoidan via lncRNA expression modulation in HepG2 cells. Cytotechnology 71:363-374 (2019). PubMed: 30632031
- Mochizuki S et al. Effect of ADAM28 on carcinoma cell metastasis by cleavage of von Willebrand factor. J Natl Cancer Inst 104:906-22 (2012). Mouse . PubMed: 22636800