Beta Lactamase Activity Assay Kit (Colorimetric) (ab197008)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Other biological fluids, Saliva, Serum, Urine
- Sensitivity: 0.06 mU/well
Overview
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Product name
Beta Lactamase Activity Assay Kit (Colorimetric)
See all Beta lactamase kits -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum, Other biological fluids -
Assay type
Enzyme activity -
Sensitivity
0.06 mU/well -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Beta Lactamase Activity Assay Kit (Colorimetric) ab197008 is a simple and sensitive assay that can detect and quantify the enzymatic activity of beta lactamases, a large family of hydrolases.
The beta lactamase assay protocol is based on the hydrolysis of the substrate Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (detectable at OD=490 nm), which is directly proportional to the amount of beta-lactamase activity.
This product can detect as low as 0.06 mU of beta-lactamase activity from bacterial samples or from biological fluids infected with beta-lactamase expressing bacteria.
Beta lacatmase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode every 2-3 min for 30-60 min
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K803 Beta-Lactamase Activity Colorimetric Assay Kit. K803-100 is the same size as the 100 test size of ab197008.
Beta Lactamases (β-Lactamases, βLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. βLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as β-lactams.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Beta Lactamase Assay Buffer 1 x 27ml Beta Lactamase Hydrolysis Buffer 1 x 100µl Nitrocefin 1 x 220µl Positive Control 1 vial -
Alternative names
- blaZ
- Penicillinase
Images
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Nitrocefin Standard Curve.
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Beta-Lactamase Activity.Beta-Lactamase activity in E. coli culture (5 µl), contaminated media (CM; 30 µl) and Positive Control (4 µl).
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Beta-Lactamase Activity.Beta-Lactamase Activity of E.coli and contaminated media expressed per milligram of protein.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197008 has not yet been referenced specifically in any publications.