Calcium Detection Kit (Cell-Based) (ab284545)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Flow cytometry-fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 2 hr
Overview
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Product name
Calcium Detection Kit (Cell-Based)
See all Calcium kits -
Detection method
Flow cytometry-fluorescent -
Assay type
Cell-based (qualitative) -
Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Product overview
This Calcium Staining Kit (ab284545) (previously called EZCell Calcium Detection Kit K432) contains membrane permeable, intracellular calcium probe. Upon cell entry, the calcium probe is hydrolyzed by intracellular esterases rendering it membrane impermeable. The cleaved calcium probe binds to intracellular calcium generating fluorescence. The intensity of fluorescence is directly proportional to intracellular calcium levels. A Positive Control reagent is also provided, and serves as experimental control, which elevates intracellular Calcium levels resulting in increased calcium staining.
This easy-to-use, non-radioactive kit allows studying the regulation of calcium at the cellular level by using Fluorescence Microscopy and Flow Cytometry in cultured cells.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K432 EZCell™ Calcium Detection Kit (Cell-Based). K432-50 is the same size as the 50 test size of ab284545.
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Tested applications
Suitable for: Flow Cyt (Intra), Fluorescent Cell Imagingmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 50 tests Assay Buffer (50X) Brown cap 2 x 1.8ml Calcium Probe Clear Cap 1 vial Experimental Control (1000X) Yellow cap 1 x 40µl -
Research areas
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Relevance
Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. -
Alternative names
- Ca++
- Ca2+
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Fluorescent Cell Imaging |
Use at an assay dependent concentration.
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| Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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Fluorescent Cell Imaging
Use at an assay dependent concentration. |
Images
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EZCell™ Calcium Detection Kit (Cell-Based)
1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µL of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol.
Fluorescence microscope images of Positive Control Staining (basal calcium staining; left panel) and Cells treated with Experimental Control reagent (middle panel). Increased fluorescence induced by Positive Control reagent compared to the Basal Calcium levels confirms intracellular accumulation of Calcium in the cells. Right panel high resolution images of cells treated with Positive Control reagent.
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EZCell™ Calcium Detection Kit (Cell-Based)1x106 Jurkat or 1x105 HeLa cells were treated with vehicle or Positive Control reagent for 30 minutes. Followed by washes, cells were incubated in 250 µL of medium + 0.5% FBS with 1X Intracellular Calcium Staining for 45 minutes at 37°C, according to kit’s protocol.
Histograms from flow analysis of Negative Control cells (black; unstained cells); Positive Control cells stained with 1X Calcium Staining (green; basal calcium staining); Experimental Control Cells treated with Positive Control Reagent (pink). Increased green fluorescence induced by Positive Control Reagent compared to the basal levels confirms increased concentration of intracellular calcium.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284545 has not yet been referenced specifically in any publications.