Key features and details
- Assay type: Competitive
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture media, Cell Lysate, Plasma, Tissue Extracts, Urine
- Sensitivity: 1 nM
Product namecAMP Assay Kit (Competitive ELISA, Fluorometric)
See all cAMP kits
Sample typeUrine, Plasma, Tissue Extracts, Cell Lysate, Cell culture media
Assay time3h 00m
cAMP Assay Kit ab138880 uses a sensitive fluorometric competitive ELISA method to quantify cAMP (cyclic AMP) levels in samples from mammals and other species.
The cAMP assay protocol uses a 96-well plate supplied coated with an anti-cAMP antibody.
HRP-labeled cAMP, and free cAMP within a sample, are added to each well of the plate. The cAMP assay is based on the competition between the HRP-labeled cAMP and the free cAMP for the fixed number of cAMP antibody binding sites on the plate.
The more free cAMP is present, the less HRP-labeled cAMP is bound. After a washing step, the amount of bound HRP-labeled cAMP is detected using a fluorometric HRP substrate. The level of signal is compared to a standard curve for free cAMP, which is run at the same time as the samples.
Unlike other cAMP assay protocols, this kit does not require an acetylation step to achieve the maximum sensitivity.
This product was previously called cAMP Direct Immunoassay Detection Kit (Fluorometric).
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 10 x 96 tests 10X Wash Solution 1 x 10ml 1 x 100ml AbRed Indicator 1 vial 1 vial Anti-cAMP-coated 96-well plate 1 unit 1 x 10 units Assay Buffer 1 x 20ml 1 x 100ml cAMP Standard 1 x 33µg 1 x 33µg Cell Lysis Buffer 1 x 10ml 1 x 100ml HRP-cAMP Conjugate 1 vial 1 vial Hydrogen Peroxide Solution 1 x 50µl 1 x 250µl Substrate Buffer 1 x 10ml 1 x 100ml
RelevanceAdenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. It plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals.
- Adenosine 3' 5' cyclic monophosphate
- Adenosine 3’ 5’ cyclic monophosphate
- Cyclic adenosine monophosphate
- Cyclic AMP
- 8-(4-Chlorophenylthio)adenosine 3',5'-cyclic monophosphate (8-CPT-cAMP), Cell permeable cAMP analog. (ab120424)
- Adenosine 3prime,5prime-cyclic monophosphate (cAMP), Second messenger. PKA activator. (ab120493)
- 8-Br-cAMP (8-Bromo-cAMP), PKA activator (ab141448)
- RHC-80267, diacylglycerol lipase inhibitor (ab141758)
cAMP dose response was measured with ab138880 in a solid black 96-well plate with a fluorescence microplate reader. The kit can detect as low as 1 nM cAMP in a 100 µl reaction volume.
cAMP dose response in various amounts of HeLa cells after and without forskolin treatment (100 micromolar - ab120058)
cAMP dose response in various amounts of 3T3-L1 cells after and without forskolin treatment (100 micromolar - ab120058)
cAMP concentration (nM) measured in various biological fluids following assay protocol.
ab138880 has been referenced in 15 publications.
- Balakrishnan A et al. Unraveling the hidden temporal range of fast ß2-adrenergic receptor mobility by time-resolved fluorescence. Commun Biol 5:176 (2022). PubMed: 35228644
- Gao R et al. Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosis. Exp Mol Med 54:1201-1213 (2022). PubMed: 35999453
- Luo Z et al. Extracellular ATP and cAMP signaling promote Piezo2-dependent mechanical allodynia after trigeminal nerve compression injury. J Neurochem 160:376-391 (2022). PubMed: 34757653
- Lacalle E et al. Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts. Animals (Basel) 12:N/A (2022). PubMed: 35454259
- Yan Y et al. Hepatic thyroid hormone signalling modulates glucose homeostasis through the regulation of GLP-1 production via bile acid-mediated FXR antagonism. Nat Commun 13:6408 (2022). PubMed: 36302774