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Cathepsin G Activity Assay Kit (Colorimetric) (ab126780)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)
  • Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)
  • Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
  • Functional Studies - Cathepsin G Activity Assay Kit (ab126780)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell Lysate, Serum, Tissue Extracts

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Overview

  • Product name

    Cathepsin G Activity Assay Kit (Colorimetric)
    See all Cathepsin G kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Tissue Extracts, Cell Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Range

    0 nmol/well - 50 nmol/well
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Cathepsin G Activity Assay Kit (Colorimetric) (ab126780) is an assay where Cathepsin G will cleave the substrate and release the dye group, p-NA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test for the activity of Cathepsin G.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K146 Cathepsin G Activity Colorimetric Assay Kit. K146-100 is the same size as the 100 test size of ab126780.

    Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Assay Buffer WM 1 x 25ml
    Cathepsin G Inhibitor, cathepsin G inhibitor Blue 1 x 20µl
    p-NA Standard (0.1 M) Yellow 1 x 20µl
    Positive Control Green 1 vial
    Substrate Red 1 x 200µl
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Cathepsin Assay Kits
  • Function

    Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Cell surface.
  • Target information above from: UniProt accession P08311 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CATG
    • CATG_HUMAN
    • Cathepsin G
    • Cathepsin G precursor
    • CathepsinG
    • CG
    • Ctsg
    • CTSG protein
    • MGC23078
    see all

Associated products

  • Related Products

    • Z-Phe-Phe-FMK, Cathepsin B and L inhibitor (ab141386)
    • Q-LEHD-OPh, caspase-9 inhibitor (ab142039)
    • Cathepsin G Inhibitor, cathepsin G inhibitor (ab142181)

Images

  • Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)
    Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)

    Standard curve with background signal subtracted (duplicates; +/- SD).

  • Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)
    Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)

    Cathepsin G activity in THP-1 lysates (duplicates +/- SD). Data corresponds to 1 million cells per well.

  • Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
    Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
    p-NA Standard Curve. Performed according to assay protocol.
  • Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
    Functional Studies - Cathepsin G Activity Assay Kit (ab126780)

    Assay results for 5 μl of positive control with and without an inhibitor 

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab126780? Please let us know so that we can cite the reference in this datasheet.

ab126780 has been referenced in 13 publications.

  • Hahn J  et al. Aggregated neutrophil extracellular traps resolve inflammation by proteolysis of cytokines and chemokines and protection from antiproteases. FASEB J 33:1401-1414 (2019). PubMed: 30130433
  • Campos MA  et al. The Biological Effects of Double-Dose Alpha-1 Antitrypsin Augmentation Therapy: A Pilot Study. Am J Respir Crit Care Med N/A:N/A (2019). PubMed: 30965011
  • Ochieng P  et al. Phospholipid transfer protein and alpha-1 antitrypsin regulate Hck kinase activity during neutrophil degranulation. Sci Rep 8:15394 (2018). PubMed: 30337619
  • Wen G  et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
  • Featherston T  et al. Expression and Localization of Cathepsins B, D, and G in Two Cancer Stem Cell Subpopulations in Moderately Differentiated Oral Tongue Squamous Cell Carcinoma. Front Med (Lausanne) 4:100 (2017). PubMed: 28775982
View all Publications for this product

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