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    products/assay-kits/cell-proliferation-staining-reagent-deep-red-fluorescence-cytopainter-ab176736.pdf

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Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (ab176736)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2) Submit a question References (6)

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Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736)
  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736)

Key features and details

  • Assay type: Cell-based (quantitative)
  • Platform: Flow cytometer
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter
    See all Cell viability/proliferation kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Other species
    Predicted to work with: Mammals
  • Product overview

    Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.


    The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.


    Main features of CytoLabeling Deep Red Reagent:



    • It eliminates the dye efflux drawback associated with CFSE

    • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required

    • It is retained up to 9 generations after staining

    • Fluorescence is preserved upon formaldehyde fixation


    This product can be used for imaging or flow cytometric analysis.


    Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide

  • Notes

    Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

  • Platform

    Flow cytometer

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests 1000 tests
    CytoLabeling Red Reagent 1 vial 1 x 2 vials
  • Research areas

    • Kits/ Lysates/ Other
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    • proliferation and senescence
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Cell Tracking kits
  • Relevance

    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
  • Alternative names

    • Cell Cytotoxicity
    • cell tracking

Images

  • Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736)
    Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736)

    Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).

  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736)
    Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736)

    Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1:1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab176736? Please let us know so that we can cite the reference in this datasheet.

ab176736 has been referenced in 6 publications.

  • Bueno-Fortes S  et al. A Gene Signature Derived from the Loss of CDKN1A (p21) Is Associated with CMS4 Colorectal Cancer. Cancers (Basel) 14:N/A (2021). PubMed: 35008299
  • Simchi L  et al. Novel Insights into the Role of UBE3A in Regulating Apoptosis and Proliferation. J Clin Med 9:N/A (2020). PubMed: 32455880
  • Elowsson Rendin L  et al. Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis. Int J Mol Sci 20:N/A (2019). PubMed: 31426504
  • Steinmann S  et al. DAPK1 loss triggers tumor invasion in colorectal tumor cells. Cell Death Dis 10:895 (2019). PubMed: 31772156
  • Muenzner JK  et al. Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential. J Cell Mol Med 22:6238-6248 (2018). PubMed: 30280520
  • Gurtner GC  et al. Wound repair and regeneration. Nature 453:314-21 (2008). PubMed: 18480812

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Deep Red Fluorescence (ab176736)

Average Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Using a fluorescence microscope, we tracked after HNDF cells over time by using the cytopainter. HNDF cells (0.5x10^6 cells/mL) were stained with ab176736 on Day 0. The cells were incubated with the cytopainter for 30 minutes at 37 degrees. The fluorescence signal could be detected for 10 days after cell seeding.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 31 2021

Novel Insights Into the Role of UBE3A in Regulating Apoptosis and Proliferation

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The FCAS setup was easily acquired, a very effective approach for the detection of proliferation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Yonatan Feuermann

Verified customer

Submitted Jun 04 2020

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