For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/assay-kits/cell-proliferation-staining-reagent-green-fluorescence-cytopainter-ab176735.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Cell Metabolism Kits Other Metabolism Assay
Share by email

Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter (ab176735)

  • Datasheet
  • Protocol Booklet
Reviews (2) Submit a question References (5)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735)
  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735)

Key features and details

  • Assay type: Cell-based (quantitative)
  • Platform: Flow cytometer, Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

You may also be interested in

ELISA
Product image
BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
Dye
Product image
Cell Tracking Dye Kit - Blue - Cytopainter (ab138890)
Biochemical
Product image
DIF-1, cell proliferation inhibitor (ab141638)

View more associated products

Overview

  • Product name

    Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter
    See all Cell viability/proliferation kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. The CytoLabeling Green Reagent is designed to label cells at Em = 511-525 nm excited with a blue laser at Ex = 488 nm.


    The CytoLabeling Green Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used.


    Main features of CytoLabeling Green Reagent:



    • It eliminates the dye efflux drawback associated with CFSE

    • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required

    • It is retained up to 9 generations after staining

    • Fluorescence is preserved upon formaldehyde fixation


    This product can be used for imaging or flow cytometric analysis.


    Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide

  • Notes

    Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests 1000 tests
    CytoLabeling Green Reagent 1 vial 2 vials
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Cell Viability and Senescence Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • proliferation and senescence
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Cell Tracking kits
  • Relevance

    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
  • Alternative names

    • Cell Cytotoxicity
    • cell tracking

Images

  • Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735)
    Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735)

    Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735).

  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735)
    Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735)

    Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735) and CFSE. Jurkat cells (2x106 cells/mL) were stained with ab176735 or CFSE (0.5 µM) on Day 0. The cells were passed serially at 1:1 ratio for 9 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (5)

Publishing research using ab176735? Please let us know so that we can cite the reference in this datasheet.

ab176735 has been referenced in 5 publications.

  • Akpe V  et al. Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems. Assay Drug Dev Technol 19:191-203 (2021). PubMed: 33471566
  • Lester Sequiera G  et al. Generation and Evaluation of Isogenic iPSC as a Source of Cell Replacement Therapies in Patients with Kearns Sayre Syndrome. Cells 10:N/A (2021). PubMed: 33807701
  • Akpe V  et al. Batch Effect Adjustment to Lower the Drug Attrition Rate of MCF-7 Breast Cancer Cells Exposed to Silica Nanomaterial-Derived Scaffolds. Assay Drug Dev Technol 19:46-61 (2021). PubMed: 33443468
  • Yahn SL  et al. Fibrotic scar after experimental autoimmune encephalomyelitis inhibits oligodendrocyte differentiation. Neurobiol Dis 134:104674 (2020). PubMed: 31731043
  • Nassour J  et al. Autophagic cell death restricts chromosomal instability during replicative crisis. Nature 565:659-663 (2019). PubMed: 30675059

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Cell Tracking and proliferation

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Easy to use. Ideal for tracking cell proliferation, cell distribution. Green fluorescence was preserved during Trypsinization. Good Fluorescence intensity was detected > 11 days after staining. Do not affect the viability of the cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 24 2020

Mouse sample in ICC/IF application

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The product is easy to use and works perfect. I use it in primary microglia cells, they are quite delicate but the staining didn't affected the survival of the cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 19 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.