Cell Viability Assay Kit (Fluorometric - Green) (ab112122)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
Product nameCell Viability Assay Kit (Fluorometric - Green)
See all Cell Viability kits
Sample typeAdherent cells, Suspension cells
Species reactivityReacts with: Mammals, Other species
There are a variety of parameters that can be used to monitor cell viability. The green fluorescent dye used in the kit is a hydrophobic compound. It easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly green fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The green fluorophore generated by the non-fluorescent substrate used in the kit has the spectral properties of fluorescein at Ex/Em = ~490 nm/520 nm. When well excited with the Argon Laser at 488 nm, the fluorophore emits intense green fluorescence at ~520 nm.
ab112122 provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. It can also be used with a fluorescence microscope equipped with a FITC filter set.
ab112122 provides an effective tool of labeling cells for fluorescence microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112122 is suitable for proliferating and non-proliferating cells.
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Review the cell health assay guide to learn about more kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
PlatformMicroplate reader, Fluor. microscope, Flow cyt.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 5 x 96 tests Assay Buffer 1 x 50ml CellGreen fluorescent dye 5 vials DMSO 1 x 200µl
RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
- cell tracking
CHO-K1 cell number response was measured with ab112122. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).
Datasheets and documents
ab112122 has been referenced in 1 publication.
- Kanno S et al. Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption. iScience 25:103770 (2022). PubMed: 35146387