Cell Viability Assay Kit (Fluorometric - Blue) (ab102501)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 100 cells/well
Overview
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Product name
Cell Viability Assay Kit (Fluorometric - Blue)
See all Cell viability/proliferation kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
> 100 cells/well -
Range
100 cells/well - 100000 cells/well -
Assay duration
One step assay -
Species reactivity
Reacts with: Mammals -
Product overview
This Cell Proliferation Assay Kit (Fluorometric-Blue) ab102501 (previously known as VisionBlue Quick Cell Viability Fluorometic Assay K303) provides by far the most sensitive and easiest means for quantifying cell proliferation and viability. Simply add the single reagent to cell culture, incubate and read the fluorescence intensity. The assay utilizes the redox dye resazurin which is not fluorescent, but upon reduction by viable metabolically active cells, the dye becomes highly fluorescent (Ex = 530-570 nm; Em = 590-620 nm). Therefore, viable metabolically active cells can be easily measured.
Visit our FAQs page for tips and troubleshooting. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K303 VisionBlue™ Quick Cell Viability Fluorometric Assay Kit. K303-2500 is the same size as the 2500 test size of ab102501.
Key Features:
- Simple procedure: Just Add-Incubate-Read. No washing, No solublization.
- Highly Sensitive: Detect as few as 100 cells.
- Longer Stability: Reagent is stable at 4C for at least a year.
- Safe: Non-radioactive, non-toxic, and cells can be further used for other experiments.
- Wide applications: Used in studying a variety of growth stimulations or inhibitions (e.g., by growth factors, cytokine, nutrients, anticancer drugs, apoptosis inducers/inhibitors, toxicityinducing chemicals, etc.).
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests 2500 tests VisionBlueTM Reagent 1 x 5ml 1 x 25ml -
Research areas
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Relevance
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
Alternative names
- Cell Cytotoxicity
- cell tracking
Images
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Cell Viability Assay Kit (Fluorometric - Blue)
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Cell Viability Assay Kit (Fluorometric - Blue)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab102501 has been referenced in 3 publications.
- Palla AR et al. Primary cilia on muscle stem cells are critical to maintain regenerative capacity and are lost during aging. Nat Commun 13:1439 (2022). PubMed: 35301320
- Tsiarli MA et al. Antenatal dexamethasone exposure differentially affects distinct cortical neural progenitor cells and triggers long-term changes in murine cerebral architecture and behavior. Transl Psychiatry 7:e1153 (2017). PubMed: 28608856
- Ho ATV et al. Prostaglandin E2 is essential for efficacious skeletal muscle stem-cell function, augmenting regeneration and strength. Proc Natl Acad Sci U S A 114:6675-6684 (2017). PubMed: 28607093