Cellular ROS Assay Kit (Red) (ab186027)
Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Microplate reader, Fluorescence microscope
- Assay time: 60 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cellular ROS Assay Kit (Red)
See all Oxidative Stress kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Assay time
0h 60m -
Product overview
Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells. The red dye used in the ROS assay protocol is cell-permeable and generates red fluorescence when it reacts with ROS. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments.
ab186027 provides an ultrasensitive fluorometric one-step ROS assay. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 520/605 nm and can be used to either quantify ROS levels in cells or to screen ROS inhibitors.
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Notes
Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Red Fluorescence).
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.
Related products
Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
To measure reactive oxygen species within cells, we recommend DCFDA / H2DCFDA - Cellular ROS Assay Kit ab113851. Alternative ROS assays are available in orange (ab186028), red (ab186027), and deep red (ab186029). ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates.
For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use ab139473; to assay superoxides use ab219943.
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Platform
Microplate reader, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 20ml DMSO 1 x 200µl ROS Red Dye (Lyophilized) 1 vial -
Research areas
Images
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Functional Studies - Cellular Reactive Oxygen Species Detection Assay Kit (ab186027)
Bar graph showing relative fluorescence of Reactive Oxygen Species induced in various quantities of Hela cells at various concentrations of H2O2 after 30 min. exposure time.
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Functional Studies - Cellular Reactive Oxygen Species Detection Assay Kit (ab186027)Bar graph showing relative fluorescence of Reactive Oxygen Species induced in various quantities of Hela cells at various concentrations of H2O2 after 120 min. exposure time.
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab186027 has been referenced in 41 publications.
- Chen X et al. Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis. Autophagy 19:54-74 (2023). PubMed: 35403545
- Lin YC et al. The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells. J Biol Chem 298:102110 (2022). PubMed: 35688208
- Crippa M et al. A microfluidic model of human vascularized breast cancer metastasis to bone for the study of neutrophil-cancer cell interactions. Mater Today Bio 17:100460 (2022). PubMed: 36278146
- Misri S et al. Cannabidiol Inhibits Tumorigenesis in Cisplatin-Resistant Non-Small Cell Lung Cancer via TRPV2. Cancers (Basel) 14:N/A (2022). PubMed: 35267489
- Gong F et al. Trehalose inhibits ferroptosis via NRF2/HO-1 pathway and promotes functional recovery in mice with spinal cord injury. Aging (Albany NY) 14:3216-3232 (2022). PubMed: 35400664