Cholesterol Uptake Assay Kit (ab236212)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cholesterol Uptake Assay Kit -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Product overview
Cholesterol Uptake Assay Kit (ab236212) provides a convenient tool for studying cellular cholesterol trafficking. The kit employs NBD Cholesterol, a fluorescently-tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, which increases cholesterol uptake by inhibiting trafficking of synthesized cholesterol, is included as a positive control. The kit provides enough NBD Cholesterol to test 250 samples in a 96-well format.
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Notes
Other cholesterol assay kits include:
- HDL and LDL/VLDL Cholesterol assay kit ab65390
- Cell-based Cholesterol assay kit ab133116
- Cholesterol/Cholesterol Ester assay kit ab65359
- Cholesterol Efflux assay kit ab196985 -
Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 kit Cell-Based Assay Buffer Tablet 1 tablet Cell-Based Assay NBD Cholesterol 1 x 500µl Cell-Based Assay U-18666A 1 x 100µl
Images
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U-18666A increases cholesterol uptake in Jurkat cells as measured by flow cytometry
Jurkat cells were seeded at a density of 5x105 cells/mL and incubated overnight in serum-free RPMI with U-18666A or vehicle and 20 µg/mL NBD Cholesterol in a cell culture incubator at 37°C. The next day, cells were transferred to a v-bottom plate for washing and reading on a flow cytometer. Cholesterol uptake was evaluated in the live cell gate using FlowJo analysis software. U-18666A at both 2.5 µM (green) and 1.25 µM (blue) showed a significant (p<0.05, t-test) shift in mean fluorescence as compared to the vehicle control (black) and the untreated (red) cells.
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Blocking intracellular cholesterol transport with U-18666A increases NBD cholesterol uptake -
U-18666A causes a dose-dependent increase in NBD Cholesterol uptake in Caco-2 cells, as measured on a fluorescent plate reader
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab236212 has been referenced in 3 publications.
- Katakam S et al. Necrotic debris and STING exert therapeutically relevant effects on tumor cholesterol homeostasis. Life Sci Alliance 5:N/A (2022). PubMed: 34983824
- Christodoulou A et al. TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis. J Cell Sci 133:N/A (2020). PubMed: 32694168
- Madhivanan K et al. Lowe syndrome patient cells display mTOR- and RhoGTPase-dependent phenotypes alleviated by rapamycin and statins. Hum Mol Genet 29:1700-1715 (2020). PubMed: 32391547