Citrate Assay Kit (ab83396)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.002 mM
Product nameCitrate Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity> 0.002 mM
Range0.002 mM - 10 mM
Assay time0h 40m
Citrate Assay Kit ab83396 provides a simple, sensitive and rapid means of quantifying citrate in biological samples.
In the citrate assay protocol, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product.
The citrate assay kit can detect 0.1 to 10 nmoles (~2 µM-10 mM) of citrate.
Citrate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K655 Citrate Colorimetric/Fluorometric Assay Kit. K655-100 is the same size as the 100 test size of ab83396.
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Citrate Assay Buffer WM 1 x 25ml Citrate Developer (Lyophilised) Green 1 vial Citrate Enzyme Mix (Lyophilised) Purple 1 vial Citrate Probe Red 1 x 0.2ml Citrate Standard (10 µmol) (lyophilised) Yellow 1 vial
RelevanceCitric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors.
- Citric acid
Datasheets and documents
ab83396 has been referenced in 10 publications.
- Song X et al. PDK4 dictates metabolic resistance to ferroptosis by suppressing pyruvate oxidation and fatty acid synthesis. Cell Rep 34:108767 (2021). PubMed: 33626342
- Son SM et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor. Nat Commun 11:3148 (2020). PubMed: 32561715
- Ding H et al. NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA. Cancer Cell Int 20:425 (2020). PubMed: 32884448
- Lou PH et al. Lipid Emulsion Containing High Amounts of n3 Fatty Acids (Omegaven) as Opposed to n6 Fatty Acids (Intralipid) Preserves Insulin Signaling and Glucose Uptake in Perfused Rat Hearts. Anesth Analg 130:37-48 (2020). PubMed: 31274599
- Hogan SE et al. Mesenchymal stromal cell-derived exosomes improve mitochondrial health in pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol 316:L723-L737 (2019). PubMed: 30652491
- Chan KR et al. Metabolic perturbations and cellular stress underpin susceptibility to symptomatic live-attenuated yellow fever infection. Nat Med 25:1218-1224 (2019). PubMed: 31308506
- Thaventhiran T et al. CD28 Superagonistic Activation of T Cells Induces a Tumor Cell-Like Metabolic Program. Monoclon Antib Immunodiagn Immunother 38:60-69 (2019). PubMed: 31009338
- Ren JG et al. Citrate Suppresses Tumor Growth in Multiple Models through Inhibition of Glycolysis, the Tricarboxylic Acid Cycle and the IGF-1R Pathway. Sci Rep 7:4537 (2017). Functional Studies . PubMed: 28674429
- Karlstaedt A et al. Oncometabolite d-2-hydroxyglutarate impairs a-ketoglutarate dehydrogenase and contractile function in rodent heart. Proc Natl Acad Sci U S A 113:10436-41 (2016). WB . PubMed: 27582470
- Elmaci AM et al. Clinical characteristics and metabolic abnormalities in preschool-age children with urolithiasis in southeast Anatolia. J Pediatr Urol N/A:N/A (2013). PubMed: 24314604