Citrate Assay Kit (ab83396)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.002 mM
Overview
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Product name
Citrate Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.002 mM -
Range
0.002 mM - 10 mM -
Assay time
0h 40m -
Product overview
Citrate Assay Kit ab83396 provides a simple, sensitive and rapid means of quantifying citrate in biological samples.
In the citrate assay protocol, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product.
The citrate assay kit can detect 0.1 to 10 nmoles (~2 µM-10 mM) of citrate.
Citrate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K655 Citrate Colorimetric/Fluorometric Assay Kit. K655-100 is the same size as the 100 test size of ab83396.
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Citrate Assay Buffer WM 1 x 25ml Citrate Developer (Lyophilised) Green 1 vial Citrate Enzyme Mix (Lyophilised) Purple 1 vial Citrate Probe Red 1 x 0.2ml Citrate Standard (10 µmol) (lyophilised) Yellow 1 vial -
Research areas
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Relevance
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors. -
Alternative names
- Citric acid
Associated products
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Related Products
Images
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Fluorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
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Citrate measured fluorimetrically in various biofluids showing concentration (micromolar).
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Citrate standard curve generated using this kit protocol
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab83396 has been referenced in 12 publications.
- Song X et al. PDK4 dictates metabolic resistance to ferroptosis by suppressing pyruvate oxidation and fatty acid synthesis. Cell Rep 34:108767 (2021). PubMed: 33626342
- Nagandla H et al. Isoform-specific Activities of Androgen Receptor and its Splice Variants in Prostate Cancer Cells. Endocrinology 162:N/A (2021). PubMed: 33300995
- Son SM et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor. Nat Commun 11:3148 (2020). PubMed: 32561715
- Ding H et al. NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA. Cancer Cell Int 20:425 (2020). PubMed: 32884448
- Lou PH et al. Lipid Emulsion Containing High Amounts of n3 Fatty Acids (Omegaven) as Opposed to n6 Fatty Acids (Intralipid) Preserves Insulin Signaling and Glucose Uptake in Perfused Rat Hearts. Anesth Analg 130:37-48 (2020). PubMed: 31274599