Cleaved Caspase-3 Staining Kit (Red) (ab65617)
Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Assay time: 2 hr
- Sample type: Adherent cells, Suspension cells
Product nameCleaved Caspase-3 Staining Kit (Red)
See all Caspase-3 kits
Sample typeAdherent cells, Suspension cells
Assay typeEnzyme activity
Assay time2h 00m
Cleaved Caspase-3 Staining Kit (Red) ab65617 provides a convenient means for detecting activated Caspase-3 in living cells. The assay utilizes the Caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.
This product is manufactured by BioVision, an Abcam company and was previously called K193 CaspGLOW™ Red Active Caspase-3 Staining Kit. K193-100 is the same size as the 100 test size of ab65617.
PlatformMicroplate reader, Fluor. microscope, Flow cyt.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Red-DEVD-FMK 1 x 100µl Wash Buffer 2 x 100ml Z-VAD-FMK 1 x 10µl
RelevanceThe caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as an inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (e.g. mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
- Apopain precursor
Datasheets and documents
ab65617 has been referenced in 5 publications.
- Chen YQ et al. Knockdown of lncRNA TTTY15 alleviates myocardial ischemia-reperfusion injury through the miR-374a-5p/FOXO1 axis. IUBMB Life 73:273-285 (2021). PubMed: 33296140
- Lien TS et al. Dengue Virus Envelope Protein Domain III Induces Nlrp3 Inflammasome-Dependent NETosis-Mediated Inflammation in Mice. Front Immunol 12:618577 (2021). PubMed: 33815373
- Di Franco S et al. CHK1 inhibitor sensitizes resistant colorectal cancer stem cells to nortopsentin. iScience 24:102664 (2021). PubMed: 34169240
- Vats K et al. Keratinocyte death by ferroptosis initiates skin inflammation after UVB exposure. Redox Biol 47:102143 (2021). PubMed: 34592565
- Di Franco S et al. FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/ß-catenin signaling pathway reporter. STAR Protoc 2:100880 (2021). PubMed: 34712995