Complex I Immunocapture Kit (ab109711)
Key features and details
- Assay type: Quantitative
Product nameComplex I Immunocapture Kit
See all Complex I kits
Species reactivityReacts with: Mouse, Rat, Cow, Human
Optimized for isolation of Complex I from small amounts of tissue for activity assays and other analyses. Based on a highly specific antibody.
250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I from heart mitochondria.
ab109711 allows for isolation of Complex I from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains NADH-ferricyanide, NADH-CoQ1 and NADH hexaaminoruthenium reductase activities after isolation, and with added lipids it also shows significant rotenone sensitivity. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Uses for ab109711 include but are not limited to examining alterations of Complex I subunits in inherited mitochondrial diseases, Parkinson's disease, Alzheimer's disease, ALS, schizophrenia, and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent (lauryl maltoside, ab109857).
Product supplied as a 6.25% slurry. For the 250 µg, this consists of 250 µg antibody conjugated to 25 µL beads in a volume of 250 µL.
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 µg Complex 1 monoclonal antibody coupled agarose beads 2 x 250µg
- NADH dehydrogenase
Datasheets and documents
ab109711 has been referenced in 24 publications.
- Molina-Granada D et al. Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit. Commun Biol 5:620 (2022). PubMed: 35739187
- D'Angelo L et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309
- Pantner Y et al. DJ-1 attenuates the glycation of mitochondrial complex I and complex III in the post-ischemic heart. Sci Rep 11:19408 (2021). PubMed: 34593886
- Peruzzo R et al. Insight into the mechanism of cytotoxicity of membrane-permeant psoralenic Kv1.3 channel inhibitors by chemical dissection of a novel member of the family. Redox Biol 37:101705 (2020). PubMed: 33007503
- Green JC et al. Characterization and Discovery of a Selective Small-Molecule Modulator of Mitochondrial Complex I Targeting a Unique Binding Site. J Med Chem 63:11819-11830 (2020). PubMed: 32945676