Complex II Immunocapture Kit (ab109799)
Key features and details
- Assay type: Quantitative
Product nameComplex II Immunocapture Kit
See all Complex II kits
Species reactivityReacts with: Mouse, Rat, Cow, Human
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria. Isolated mitochondria are available from several species for use as control samples.
The Complex II Immunocapture Kit allows isolation of the succinate dehydrogenase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for the Complex II immunocapture kit include research on aging genetic mitochondrial disease, and various cancers due to mutations in this enzyme complex such as paragangliomas and phaeo-chromocytomas.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 µg monoclonal Complex II antibody irreversibly cross-linked to protein G-agarose beads 1 x 250µg
RelevanceComplex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.
- Succinate coenzyme Q reductase
- Succinate dehydrogenase
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
Complex II immunoprecipitation using antibody ab109865 crosslinked to protein G-agarose beads as product ab109799. Complex II was immunoprecipitated from a lauryl maltoside detergent extract of 1000 ug bovine heart mitochondria or 500 ug human heart mitochondria. The gel was stained with coomassie brilliant blue.
Datasheets and documents
ab109799 has been referenced in 7 publications.
- Jaña F et al. Complex I and II are required for normal mitochondrial Ca2+ homeostasis. Mitochondrion 49:73-82 (2019). PubMed: 31310854
- Fernández-Tussy P et al. miR-873-5p targets mitochondrial GNMT-Complex II interface contributing to non-alcoholic fatty liver disease. Mol Metab 29:40-54 (2019). PubMed: 31668391
- Garcia-Diaz B et al. Infantile encephaloneuromyopathy and defective mitochondrial translation are due to a homozygous RMND1 mutation. Am J Hum Genet 91:729-36 (2012). PubMed: 23022099
- Finley LW et al. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS One 6:e23295 (2011). IP ; Mouse . PubMed: 21858060
- Paddenberg R et al. Impact of modulators of mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) on hypoxic pulmonary vasoconstriction. Adv Exp Med Biol 648:361-8 (2009). PubMed: 19536500
- Ahn BH et al. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A 105:14447-52 (2008). PubMed: 18794531
- Schilling B et al. Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria. Biochim Biophys Acta 1762:213-22 (2006). PubMed: 16120479