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    products/assay-kits/complex-iv-immunocapture-kit-ab109801.pdf

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Signal Transduction Metabolism Mitochondrial
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Complex IV Immunocapture Kit (ab109801)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (3)References (11)

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Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)

    Key features and details

    • Assay type: Quantitative

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    Overview

    • Product name

      Complex IV Immunocapture Kit
      See all Complex IV kits
    • Assay type

      Quantitative
    • Species reactivity

      Reacts with: Cow, Human
    • Product overview

      ab109801 Complex IV Immunocapture Kit allows isolation of the cytochrome c oxidase complex from small amounts of tissue.


      250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.


      This kit facilitates subsequent analysis of both assembly state and accrued post-translational modifications of all 13 subunits of Complex IV. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109801 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.


       


      Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).


       

    • Notes

      Related products

      Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 250 µg
      Monoclonal Complex IV antibody irreversibly crosslinked to protein G-agarose beads  1 x 250µg
    • Research areas

      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Other Metabolism Assay
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
      • Kits/ Lysates/ Other
      • Kits
      • Immunocapture
      • OXPHOS
    • Alternative names

      • cco
      • COX
      • Cytochrome c oxidase
      • Cytochrome oxidase
    • Database links

      • Entrez Gene: 4512 Human
      • Entrez Gene: 4513 Human
      • Entrez Gene: 4514 Human
      • Entrez Gene: 1345 Human
      • Entrez Gene: 1351 Human
      • Entrez Gene: 1329 Human
      • Entrez Gene: 1349 Human
      • Entrez Gene: 1337 Human
      • Entrez Gene: 1327 Human
      • Entrez Gene: 1340 Human
      • Entrez Gene: 1350 Human
      • Entrez Gene: 9377 Human
      • Entrez Gene: 1346 Human
      • SwissProt: P00395 Human
      • SwissProt: P00403 Human
      • SwissProt: P00414 Human
      • SwissProt: P13073 Human
      • SwissProt: P12074 Human
      • SwissProt: P15954 Human
      • SwissProt: P24311 Human
      • SwissProt: P10176 Human
      • SwissProt: P14854 Human
      • SwissProt: P09669 Human
      • SwissProt: P24310 Human
      • SwissProt: P20674 Human
      • SwissProt: P10606 Human
      see all

    Associated products

    • Related Products

      • 10% Lauryl Maltoside Solution (ab109857)
      • Anti-Complex IV Immunocapture antibody [31E91B82G9] (ab109863)

    Images

    • Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)
      Immunoprecipitation - Complex IV Immunocapture Kit (ab109801)
      Complex IV immunoprecipitation using antibody ab109863/MS101c crosslinked to protein G-agarose beads as product ab109801

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (11)

    Publishing research using ab109801? Please let us know so that we can cite the reference in this datasheet.

    ab109801 has been referenced in 11 publications.

    • Thiriveedi VR  et al. Glutathionylated and Fe-S cluster containing hMIA40 (CHCHD4) regulates ROS and mitochondrial complex III and IV activities of the electron transport chain. Redox Biol 37:101725 (2020). PubMed: 32971361
    • Wright JN  et al. Acute increases in O-GlcNAc indirectly impair mitochondrial bioenergetics through dysregulation of LonP1-mediated mitochondrial protein complex turnover. Am J Physiol Cell Physiol 316:C862-C875 (2019). PubMed: 30865517
    • Morscher RJ  et al. Mitochondrial translation requires folate-dependent tRNA methylation. Nature 554:128-132 (2018). PubMed: 29364879
    • Oliva CR  et al. Identification of Small Molecule Inhibitors of Human Cytochrome c Oxidase That Target Chemoresistant Glioma Cells. J Biol Chem 291:24188-24199 (2016). IP . PubMed: 27679486
    • Desmurs M  et al. C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization. Mol Cell Biol 35:1139-56 (2015). PubMed: 25605331
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Customer kindly contacted us to determine if this product might work with zebrafish or goldfish samples.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2012

    Answer

    Thank you for contacting us.

    I have been in contact with the lab regarding species reactivity of this product as you have requested. They have tested the paired antibody in zebrafish and this will definitely not work on Zebrafish or goldfish.

    While not IP or immunocapture some of the mitosciences antibodies were used in a mitochondrial zebrafish project:

    http://www.ncbi.nlm.nih.gov/pubmed?term=17761683

    Should you wish to test any Abcam products in an untested species or application we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2012

    Question

     I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 30 2011

    Answer

    The MitoSciences laboratory recommends the following: Use 1 mg of brain tissue lysate, eluting into the minimum volume possible. A glycine buffer elution procedure allows the reuse of the beads. If you wish to analyze the product by Western blot, we can scale back the amount of material required. So using whole tissue I think you can be very confident using 1 mg of tissue or less. However, be aware of possible mouse capture antibody elution from the beads into the sample when blotting. Ways to resolve this problem: (1) Do not include reducing agent in sample during electrophoresis. This should maintain eluted antibody at 200 kDa during electrophoresis. (2) Western blotting – Blot with rabbit antibodies or use a secondary detection system that recognizes only the native mouse antibody being used to Western blot.

    Read More

    Abcam Scientific Support

    Answered on Sep 30 2011

    Question

     I would like to ask what is the minimum amount of mouse brain tissue required for Immunocapture kit Complex I (as well as complex III, and IV) so as to pull down Complex I. We would need enough material for running an SDS gel.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 29 2011

    Answer

    Thank you for contacting us. The protocol recommends 300 ug minimum and 5 mg ideally, but these figures refer to isolated mitochondria, and not intact tissue. I have asked the laboratory to send this information, the minimum amount of tissue required, and I will forward this when I receive it. If you are interested in isolating mitochondria, which will make the immunocapture more efficient and the yield more pure, I recommend considering the mitochondria isolation kits, ab110168 and ab110169.

    Read More

    Abcam Scientific Support

    Answered on Sep 29 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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