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Complex IV Rodent Immunocapture Kit (ab109860)

Submit a review Q&A (4)References (1)

Overview

  • Product name

    Complex IV Rodent Immunocapture Kit
    See all Complex IV kits

  • Species reactivity

    Reacts with: Mouse, Rat

  • Product overview

    Complex IV Rodent Immunocapture Kit ab109860 allows for isolation of the cytochrome c oxidase complex from small amounts of tissue. 


    250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.


     This kit facilitates the analysis of both assembly state and accrued post-translational modifications of all 13 subunits of the cytochrome c oxidase complex. The immunoprecipitated Complex IV shows cytochrome c oxidase activity while bound to the beads and this activity is fully sensitive to cyanide. Uses for ab109860 include but are not limited to examining alterations of Complex IV subunits in inherited mitochondrial diseases, Alzheimer's disease, schizophrenia and cancer.


     


    Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).


     

  • Notes

    Related products

    Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

Properties

Associated products

References (1)

Publishing research using ab109860? Please let us know so that we can cite the reference in this datasheet.

ab109860 has been referenced in 1 publication.

  • Strub GM  et al. Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration. FASEB J 25:600-12 (2011). PubMed: 20959514

Customer reviews and Q&As

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Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

Hello.
I have purchased the product: Complex IV Rodent Immunocapture Kit. After several experiemnts with their
respective controls we have conclude that is not working. May be during the delivery proccess the Kit
have been spoiled or damaged as Complex I Immunocapture Kit (also purchase to Mitoscience) works nicely.
Please, consider to send us other kit.

Read More
Answer

I am very sorry but, based on the lot number you provided, it appears you receivedthe IP reagent for ab109801, which only works with HUMAN and COW samples. Youunfortunately did not receive the IP reagent forab109860, a complex IV immunocapture kit suitable for RODENT SAMPLES.

Of course I will be happy to send youfree replacement kitforuse with your rodent samples,ab109860. If you could, please provide thepurchase order number or Abcam order reference number associated with the purchase of this kit so I can process the replacement.

Thanks for your patience with our troubelshooting process and again I am very sorry for this mistake. We will get you the newkit as soon as possible.

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Question

Hello. I have purchased the product: Complex IV Rodent Immunocapture Kit. After several experiemnts with their respective controls we have conclude that is not working. May be during the delivery proccess the Kit have been spoiled or damaged as Complex I Immunocapture Kit (also purchase to Mitoscience) works nicely. Please, consider to send us other kit.

Read More
Answer

I am very sorry to hear there is a problem here. If you could, a few additional details would be helpful to help us determine what the problems may be: 1. Canyou addmolecular weight markers and show the full gel so we can see the top of the blot. 2.What is the lot number of this kit (so we can check the QC data)? 3. Some protocol details, including: a. How sample was prepped? b. Is input the sample before extraction or post extraction? c. How were the beads eluted? Thanks very much for the additional information!

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Question

Hello. I attach a file from IP experiments. You`ll see how while using Complex I Immunocapture kit i succeed in Immunocapturing CI (WB against NDUFA9) but i also Immunocapture complex IV, (WB against COX4), but with Complex IV Immunocapture ki,t i Immunocapture nothing. I have used the right detergent and concentrations. I can not explain that result but thinking something is wrong with the kit.

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Answer

Thank you for sending along your IP/western blot data. It does appear the IP of  complex IV is not working. If you have purchased this kit within the last 6 months or so I would be happy to send you a new kit. So I can process your request, please include the PO number or Abcam order reference number associated with this order.  

Read More

Question

Hello. I have purchased the product: Complex IV Rodent Immunocapture Kit. After several experiemnts with their respective controls we have conclude that is not working. May be during the delivery proccess the Kit have been spoiled or damaged as Complex I Immunocapture Kit (also purchase to Mitoscience) works nicely. Please, consider to send us other kit.

Read More
Answer

Thank you for your enquiry. I'm sorry to hear the Complex IV Immunocapture kit is giving you trouble. I think it would be helpful if you could give a few more details describing how the kit is not working. Including an attached figure would be quite useful as well. Thanks in advance for the additional information.

Read More

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