D-Lactate Assay Kit (Colorimetric) (ab83429)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mM
Product nameD-Lactate Assay Kit (Colorimetric)
See all D-Lactate kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity> 0.01 mM
Range0.01 mM - 10 mM
Assay time0h 40m
D-Lactate Assay Kit (Colorimetric) Kit ab83429 provides a fast, easy way to accurately measure D-lactate in a variety of biological samples.
In the D-lactate assay protocol, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media.
D-lactate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K667 D-Lactate Colorimetric Assay Kit. K667-100 is the same size as the 100 test size of ab83429.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests D-Lactate Assay Buffer WM 1 x 25ml D-Lactate Enzyme Mix (Lyophilised) Green 1 vial D-Lactate Standard (100 mM) Yellow 1 x 100µl D-Lactate Substrate Mix (Lyophilised) Red 1 vial
RelevanceD-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.
- D-Lactic acid
Measurement of plasma D-lactate at 24h and 48h after ANP induction using ab83429.
To evaluate the severity of intestinal barrier dysfunction, plasma DAO and D-lactate were measured as indicators of intestinal mucosal mass and integrity which can reflect the extent of permeability and damage in the intestine.
Compared with the SO groups D-lactate significantly increased in the ANP groups at 24h and 48h (all p<0.05). The ANP48h group had significantly higher levels of plasma D-lactate than those of the ANP24h group (p<0.05, respectively).
D-Lactate measured in human serum, plasma and saliva, mouse serum and plasma and fresh yoghurt showing quantity (nmol) per ml of tested sample. Samples were diluted 3-27 fold.
Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
D-Lactate Standard Curve. The assay is performed according to the kit instruction. The assay specifically detects D-Lactate in the presence of up to 1000X L-Lactate.
Datasheets and documents
ab83429 has been referenced in 11 publications.
- Sarker MK et al. Attenuation of diabetic kidney injury in DPP4-deficient rats; role of GLP-1 on the suppression of AGE formation by inducing glyoxalase 1. Aging (Albany NY) 12:593-610 (2020). PubMed: 31905169
- Guan Y et al. Circular RNA circPITX1 knockdown inhibits glycolysis to enhance radiosensitivity of glioma cells by miR-329-3p/NEK2 axis. Cancer Cell Int 20:80 (2020). PubMed: 32190004
- Liu C et al. Silencing TCF4 Sensitizes Melanoma Cells to Vemurafenib Through Inhibiting GLUT3-Mediated Glycolysis. Onco Targets Ther 13:4905-4915 (2020). PubMed: 32581551
- Liu S et al. SPRY4-IT1 promotes survival of colorectal cancer cells through regulating PDK1-mediated glycolysis. Anim Cells Syst (Seoul) 24:220-227 (2020). PubMed: 33029299
- Lu Z et al. Functional Changes of the Community of Microbes With Ni-Dependent Enzyme Genes Accompany Adaptation of the Ruminal Microbiome to Urea-Supplemented Diets. Front Microbiol 11:596681 (2020). PubMed: 33414773