ECL Western Blotting Substrate Kit (ab65623)
Overview
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Product name
ECL Western Blotting Substrate Kit -
Product overview
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
Our ECL kits include this kit ab65623 and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit ab133406 to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K820 ECL Western Blotting Substrate Kit. K820-500 is the same size as the 500 test size of ab65623.
Reagents provided are sufficient for 400 cm2 of membrane.
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Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 50 tests 500 tests Reagent A 1 x 25ml 1 x 250ml Reagent B 1 x 25ml 1 x 250ml -
Research areas
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Relevance
ECL (enhanced luminol-based chemiluminescent) detection is a sensitive, nonradioactive method for easy detection of horseradish peroxidase (HRP) on western blots. -
Alternative names
- Enhanced luminol based chemiluminescent
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent dilution.
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| Notes |
|---|
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WB
Use at an assay dependent dilution. |
Images
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ECL Western Blotting Substrate Kit - 50 Tests (ab65623)ECL Western Blotting Substrate
Datasheets and documents
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SDS download
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Datasheet download
References (110)
ab65623 has been referenced in 110 publications.
- Chen B et al. N6‑methyladenosine‑induced long non‑coding RNA PVT1 regulates the miR‑27b‑3p/BLM axis to promote prostate cancer progression. Int J Oncol 62:N/A (2023). PubMed: 36484368
- Yan J et al. Long non-coding RNA MIR17HG sponges microRNA-21 to upregulate PTEN and regulate homoharringtonine-based chemoresistance of acute myeloid leukemia cells. Oncol Lett 23:24 (2022). PubMed: 34868361
- Lv F et al. Therapeutic exosomal vaccine for enhanced cancer immunotherapy by mediating tumor microenvironment. iScience 25:103639 (2022). PubMed: 35024580
- Jiang J et al. Circular RNA circHIPK3 is downregulated in diabetic cardiomyopathy and overexpression of circHIPK3 suppresses PTEN to protect cardiomyocytes from high glucose-induced cell apoptosis. Bioengineered 13:6272-6279 (2022). PubMed: 35200097
- Jiang L et al. LncRNA KCNQ1OT1-mediated cervical cancer progression by sponging miR-1270 as a ceRNA of LOXL2 through PI3k/Akt pathway. J Obstet Gynaecol Res 48:1001-1010 (2022). PubMed: 35218109