For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/assay-kits/edu-assay--edu-staining-proliferation-kit-ifluor-647-ab222421.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Cell Metabolism Kits Other Metabolism Assay
Share by email

EdU Assay / EdU Staining Proliferation Kit (iFluor 647) (ab222421)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (17)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

EdU Proliferation Kit (iFluor 647) (ab222421)
  • EdU Proliferation Kit (iFluor 647) (ab222421)
  • EdU Proliferation Kit (iFluor 647) (ab222421)
  • EdU Proliferation Kit (iFluor 647) (ab222421)

Key features and details

  • Assay type: Cell-based
  • Detection method: Fluorescent
  • Platform: Flow cytometer, Fluorescence microscope
  • Sample type: Adherent cells, Suspension cells

You may also be interested in

Primary
Product image
Anti-HIF-1 alpha antibody [H1alpha67] (ab1)
Assay
Product image
Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)
ELISA
Product image
BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)

View more associated products

Overview

  • Product name

    EdU Assay / EdU Staining Proliferation Kit (iFluor 647)
    See all Cell viability/proliferation kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    EdU Assay / EdU Staining Proliferation Kit (iFluor 647) ab222421 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy. The iFluor 647 dye (Ex/Em: 649/664 nm) has spectral properties almost identical to those of Cy5®and alternative red fluorophores.


    EdU staining protocol summary (wash cells between each step):
    - add EdU solution to cells to be stained
    - incubate cells for 2-4 hrs under optimal growth conditions
    - add fixative solution and incubate for 15 min
    - add permeabilization buffer and incubate for 15/20 min
    - add reaction mix to fluorescently label EdU and incubate for 30 min
    - analyze with flow cytometer / fluorescence microscope


    EdU staining can also be combined with antibody staining or cell staining with other fluorescent dyes.


    This kit provides enough reagents to perform 50 flow cytometry tests or 50 microscopy tests (for 18 x 18 mm coverslips) or 200 microscopy tests (adapted for 96-well plate format).

  • Notes

    Previously called EdU Proliferation Assay Kit (iFluor 647).

    The most accurate method to measure DNA proliferation is by directly measuring DNA synthesis. The most common method for this uses antibody-based detection of the nucleoside analog bromo-deoxyuridine (BrdU). 

    EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog that is an alternative to BrdU, is also used in DNA proliferation assays that are simpler and faster than the BrdU assay. NB: EdU is also available as free molecule as ab146186 (EdU).

    In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample. A fluorescent azide, such as iFluor-488, is then added. The fluorescent azide is small enough to diffuse freely through native tissues and DNA, and it covalently cross-links to the EdU in a 'click' chemistry reaction. 

    The main advantages of EdU staining over using BrdU are:
    - no harsh DNA hydrolysis / DNA denaturing step is required with EdU staining (unlike in the BrdU assay where it is used to give the BrdU antibody access to BrdU within the DNA)
    - EdU staining is faster, and has less steps, than BrdU staining

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    10X Permeabilization Buffer 1 x 25ml
    Azide iFluor 647 Dye (500 µM) 1 x 130µl
    Copper Sulfate (100 mM) 1 x 1ml
    Dimethylsulfoxide (DMSO) 1 x 4.25ml
    EdU 1 x 10mg
    Fixative (40% formaldehyde solution) 1 x 5ml
    Sodium Ascorbate 1 x 400mg
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Cell Viability and Senescence Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • proliferation and senescence
  • Relevance

    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
  • Alternative names

    • Cell Cytotoxicity
    • cell tracking

Associated products

  • Related Products

    • EdU Assay / EdU Staining Proliferation Kit (iFluor 488) (ab219801)

Images

  • EdU Proliferation Kit (iFluor 647) (ab222421)
    EdU Proliferation Kit (iFluor 647) (ab222421)

    Dot plot of EdU-647 staining (Y-axis, 647) vs FSC. 106 HeLa cells were incubated with the stated concentrations of EdU for 3 hours. Control cells (next image) were incubated with media only. Images were acquired on an Accuri C6 Cytometer (BD Biosciences) with cells excited using a 640 nm laser and data analyzed using FlowJo (v10). The percentage of gated cells (EdU positive) is highlighted.

  • EdU Proliferation Kit (iFluor 647) (ab222421)
    EdU Proliferation Kit (iFluor 647) (ab222421)

    Dot plot of EdU-647 staining (Y-axis, 647) vs FSC. 106 HeLa cells were incubated with the stated concentrations of EdU for 3 hours. This image shows control cells, incubated with media only. Images were acquired on an Accuri C6 Cytometer (BD Biosciences) with cells excited using a 640 nm laser and data analyzed using FlowJo (v10). The percentage of gated cells (EdU positive) is highlighted.

  • EdU Proliferation Kit (iFluor 647) (ab222421)
    EdU Proliferation Kit (iFluor 647) (ab222421)

    EdU staining of proliferating cells. HeLa cells (4 x 104 cells/well in 96 plate) were incubated with 20 μM EdU for 3 hours. Cells were analyzed using a TCS SP8 confocal microscope (Leica-Microsystems). DNA (blue) was staining with Hoechst 33342 (ab145597). Purple cells show EdU/Hoechst-positive cells.

  • EdU Proliferation Kit (iFluor 647) (ab222421)
    EdU Proliferation Kit (iFluor 647) (ab222421)

    EdU staining of proliferating cells. HeLa cells (4 x 104 cells/well in 96 plate) were incubated with 10 μM EdU for 3 hours. Cells were analyzed using a TCS SP8 confocal microscope (Leica-Microsystems). DNA (blue) was staining with Hoechst 33342 (ab145597). Purple cells show EdU/Hoechst-positive cells.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab222421? Please let us know so that we can cite the reference in this datasheet.

ab222421 has been referenced in 17 publications.

  • Shu HY  et al. Trimetazidine enhances myocardial angiogenesis in pressure overload-induced cardiac hypertrophy mice through directly activating Akt and promoting the binding of HSF1 to VEGF-A promoter. Acta Pharmacol Sin 43:2550-2561 (2022). PubMed: 35217815
  • Chu XD  et al. Thrombospondin-2 holds prognostic value and is associated with metastasis and the mismatch repair process in gastric cancer. BMC Cancer 22:250 (2022). PubMed: 35255858
  • Shen HY  et al. The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects. J Oncol 2022:2800488 (2022). PubMed: 35422863
  • Wang Z  et al. FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance. Anal Cell Pathol (Amst) 2022:9675466 (2022). PubMed: 35498155
  • Ji T  et al. A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641. J Immunol Res 2022:5582811 (2022). PubMed: 35600049
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

EdU staining in epithelial cells

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Epithelial cells were grown with MEM medium supplement with 10%FBS. Half of the medium was replaced with EdU solution and cells were incubated 24h.
Cells were fixed with 4%PFA 15min RT and the staining was performed following the manufactures instructions. Nuclei counterstained with Hoeschst 33342 (1/2000).
Imagen acquired with confocal microscope.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 31 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.