For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/assay-kits/er-staining-kit-red-fluorescence-cytopainter-ab139482.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Cell Staining Kits Endoplasmic Reticulum Staining Kits
Share by email

ER Staining Kit - Red Fluorescence - Cytopainter (ab139482)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (4)Q&A (1)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Fluorescent staining using CytoPainter ER Staining kit
  • Fluorescence excitation and emission spectra for CytoPainter ER Staining kit

You may also be interested in

Kit
Product image
Nucleolar Staining Kit - Green Fluorescence - Cytopainter (ab139475)
Dye
Product image
Golgi Staining Kit - Green Fluorescence - Cytopainter (ab139483)
ELISA
Product image
Mouse IL-13 ELISA Kit (ab219634)

View more associated products

Overview

  • Product name

    ER Staining Kit - Red Fluorescence - Cytopainter
    See all Endoplasmic reticulum kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the red dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. (Ex/Em = 580/677 nm). 


    One important application of the ER staining kit is in fluorescence co-localization imaging with green fluorescent protein (GFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.


    The ER staining kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein, or coumarin.


    A nuclear counterstain is also included in the kit.


    Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide

  • Notes

    This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the Texas Red region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.

    Previously called CytoPainter ER Staining Kit -Red Fluorescence.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 500 tests
    10X Assay Buffer 1 x 15ml
    Hoechst 33342 Nuclear Stain 1 x 50µl
    Red Detection Reagent 1 x 50µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Endoplasmic Reticulum Staining Kits
  • Alternative names

    • ER

Images

  • Fluorescent staining using CytoPainter ER Staining kit
    Fluorescent staining using CytoPainter ER Staining kit
    Live HeLa cells stained with Red Detection Reagent (A), Hoechst dye (B) and resulting composite image (C).
  • Fluorescence excitation and emission spectra for CytoPainter ER Staining kit
    Fluorescence excitation and emission spectra for CytoPainter ER Staining kit
    Fluorescence excitation and emission spectra for Red dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab139482? Please let us know so that we can cite the reference in this datasheet.

ab139482 has been referenced in 10 publications.

  • Zhang X  et al. Structural and functional analysis of the roles of influenza C virus membrane proteins in assembly and budding. J Biol Chem 298:101727 (2022). PubMed: 35157850
  • Deng L  et al. Macrophages take up VLDL-sized emulsion particles through caveolae-mediated endocytosis and excrete part of the internalized triglycerides as fatty acids. PLoS Biol 20:e3001516 (2022). PubMed: 36026438
  • Jang H  et al. FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress. Cell Death Dis 12:407 (2021). PubMed: 33859174
  • Feng D  et al. Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Sci Adv 7:N/A (2021). PubMed: 33990333
  • Khan ES  et al. Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro. BMC Mol Cell Biol 21:22 (2020). PubMed: 32228452
  • Mao F  et al. Hemocyte phagosomal proteome is dynamically shaped by cytoskeleton remodeling and interorganellar communication with endoplasmic reticulum during phagocytosis in a marine invertebrate, Crassostrea gigas. Sci Rep 10:6577 (2020). PubMed: 32313134
  • Bilska A  et al. Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C. Nat Commun 11:2032 (2020). PubMed: 32341344
  • Garufi A  et al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation. Biomolecules 10:N/A (2020). PubMed: 32138264
  • Janezic EM  et al. N-glycosylation of a1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis. Sci Rep 10:7209 (2020). PubMed: 32350295
  • Liu T  et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). PubMed: 29526322

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Combining ER dye and vimentin antibody staining

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
HUVEC (30000) were seeded 8-well ibiTreat ibidi culture slides, and grown until nearly confluent for 24-48h.
Cells were washed with PBS, fixated with 1% PFA for 20' RT and subsequently permeabilized with PBS/0.1%Triton-X100 for 10’RT.
Cells were incubated with primary Ab (mouse anti-vimentin, clone V9, Dako, 1:200), secondary goat-anti-mouse biotin (Dako, 1:200) and streptavidin-Alexa488 (ThermoFisher 1:500), all diluted in PBS/0.5%BSA. All incubations were for 45-60' RT in the dark, followed by 3 washes with PBS.
Cytopainter ER staining dye was diluted 1:1000 in 1x assay buffer, added to the cells, and incubated for 30' at 37C. If applicable, Hoechst was also diluted 1:1000 in the same buffer.
Cells were washed 2x in PBS before images were taken on a Leica DMIL inverted microscope equipped with a Leica DFC345FX camera, and processed in Leica Application Suite software.

Figure:
HUVEC stained with Cytopainter ER staining dye (red) Hoechst (blue) and anti-vimentin Ab (green).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Judy Van Beijnum

Verified customer

Submitted Oct 26 2017

Chicken Fibroblasts

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Chicken Embryonic Fibroblasts (CEF) stained according to Abcam's recommended protocol.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Marcelo Pelajo Machado

Verified customer

Submitted May 09 2017

Economic and very easy to use

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
1. Mouse astrocyte primary culture was fixed in 4%PFA for 5 minutes.
2. Block and permeabilized o/n, 4C with 1%BSA+0.1% Triton-X.
3. In 1X buffer: 1:1000 Texas red (ER) and 1:1000 Hoestch, room temperature for 40 minutes,
4. Rinse with 1X buffer twice and mount.
Imaged in a Leica SP8, 40X magnification, 1024x1024.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Maria Velasco

Verified customer

Submitted Mar 17 2017

ab139482 for mouse tissue

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
This kit is good!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 18 2015

Question




Product code: 139482


 


Inquiry: Hi, our group bought the cytopainter ER ab139482 to staining HeLa cells but we found the reagent being very toxic and induces huge cell detachment. We followed the protocol to dilute the reagent (warm up reagent to 37, keep cells at 37, etc), we even tried to further dilute the ER dye but we still haven’t had a good staining.

Read More

Abcam community

Verified customer

Asked on May 04 2018

Answer

Staining at low concentrations does not appear to be toxic to cells. I know that you already tried to reduce the concentration, maybe you can try to reduce it more.

Customer feedback: I've tested the ER staining on Friday and the 0.2X concentration can stain HeLa cells well without causing too much toxicity. It is good new but we spent quite some effort to obtain the same results in HeLa as you advertise on the website. In contrast, the staining using Mitochondria and lysosome Cytopainter were just as you described. We've used many of your products and this ER stain protocol was the least satisfying one so far. Hope the information could be modified and cost less optimisation time in future.

Read More

Abcam Scientific Support

Answered on May 04 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.