Fatty Acid Oxidation Assay Kit (flow cytometry) (ab118183)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Fatty Acid Oxidation Assay Kit (flow cytometry)
See all Fatty Acid Oxidation kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Product overview
Fatty Acid Oxidation Assay Kit ab118183 contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry. The assay combines the power of single cell analysis obtained from flow cytometry with the specificity of antibody-based immunostaining to quantify protein levels in cultured cells. Cells are harvested and fixed/permeabilized in suspension, targets of interest are detected indirectly with highly specific well-characterized monoclonal antibodies that are then labeled with fluorescent antibodies
This product was previously called Fatty Acid Oxidation Human Flow Cytometry Kit.
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Notes
Spin down the contents of the antibody vials upon receipt of the kit. Store all components upright at 4°C. This kit is stable for at least 6 months from receipt.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Flow cytometer
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 100X ACADM Primary Antibody 1 x 0.12ml 100X ACADVL Primary Antibody 1 x 0.12ml 100X HADHA Primary Antibody 1 x 0.12ml 100X Normal Mouse IgG 1 x 0.12ml 100X Triton X-100 1 x 1.25ml 10X Phosphate Buffered Saline 1 x 100ml 400X Tween-20 1 x 4ml Blocking Solution 1 x 25ml -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Hydrolysis
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Alternative names
- Beta Oxidation
- Beta-Oxidation
Images
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Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)
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Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)After background subtraction, the ACADVL deficient cell line shows a 93% reduction in the level of the ACADVL protein.
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Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)Expression levels of HADHA was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes HADHB:p[R61H];[R247H]. Black: Background fluorescence was determined with the supplied negative control.
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Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)After background subtraction, the LCHAD deficient cell line shows a 50% reduction in the level of the HADHA protein.
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Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)Expression levels of ACADM was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes ACADM:p[K604E] Black: Background fluorescence was determined with the supplied negative control.
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Flow Cytometry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)After background subtraction, the ACADM deficient cell line shows a 57% reduction in the level of the ACADM protein.
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Immunocytochemistry - Fatty Acid Oxidation Human Flow Cytometry Kit (ab118183)Antibody specificity demonstrated by immunocytochemistry. Cells were processes with the Flow cytometry protocol as explained above. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab118183 has been referenced in 7 publications.
- Zhang Z et al. Corticosterone Enhances the AMPK-Mediated Immunosuppressive Phenotype of Testicular Macrophages During Uropathogenic Escherichia coli Induced Orchitis. Front Immunol 11:583276 (2020). PubMed: 33363533
- Kumar N et al. Assessment of temporal functional changes and miRNA profiling of human iPSC-derived cardiomyocytes. Sci Rep 9:13188 (2019). PubMed: 31515494
- Bartel K et al. Connecting lysosomes and mitochondria - a novel role for lipid metabolism in cancer cell death. Cell Commun Signal 17:87 (2019). PubMed: 31358011
- Singh KB et al. Prostate cancer chemoprevention by sulforaphane in a preclinical mouse model is associated with inhibition of fatty acid metabolism. Carcinogenesis 39:826-837 (2018). PubMed: 29668854
- Pascual G et al. Targeting metastasis-initiating cells through the fatty acid receptor CD36. Nature 541:41-45 (2017). PubMed: 27974793
- Tabe Y et al. Bone Marrow Adipocytes Facilitate Fatty Acid Oxidation Activating AMPK and a Transcriptional Network Supporting Survival of Acute Monocytic Leukemia Cells. Cancer Res 77:1453-1464 (2017). PubMed: 28108519
- Cook KL et al. Endoplasmic Reticulum Stress Protein GRP78 Modulates Lipid Metabolism to Control Drug Sensitivity and Antitumor Immunity in Breast Cancer. Cancer Res 76:5657-5670 (2016). PubMed: 27698188