Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Other biological fluids, Plasma, Serum
- Sensitivity: 0.2 mM
Product nameFRAP Assay Kit (Ferric Reducing Antioxidant Power Assay)
Sample typeSerum, Plasma, Other biological fluids
FRAP Assay Kit ab234626 provides a quick, sensitive and easy way for measuring the antioxidant capacity of various biological samples. FRAP assay stands for Ferric Reducing Antioxidant Power Assay.
The FRAP assay is high-throughput, adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.
The assay measures the antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the samples. Following the reduction of the ferric iron, a blue color develops that is read colorimetrically at 594 nm.
The antioxidant potential of samples is determined using a ferrous iron standard curve and results are expressed as Fe2+ equivalents (µM) or FRAP value.
This product is manufactured by BioVision, an Abcam company and was previously called K515 Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric). K515-200 is the same size as the 200 test size of ab234626.
Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 200 tests FeCl3 Solution 1 x 4ml Ferrous Standard (2 mM) 1 x 1ml FRAP Assay Buffer 1 x 50ml FRAP Positive Control 1 x 0.1ml FRAP Probe 1 x 4ml
Ferrous standard curve.
Absorbance readings for positive control and 10 μl diluted solutions of grape juice (1:50 dilution with dH2O), strawberry methanolic extract (extract made from 50 mg of freeze-dried strawberries in 5 ml of extraction solvent and final solution diluted 1:80 times with dH2O) and green tea (brewed for 5 minutes and diluted 1:120 times with dH2O).
Absorbance readings for mM Ferrous equivalents or FRAP of grape juice, strawberry and green tea.
ab234626 has been referenced in 3 publications.
- Choi IS et al. Production, Characterization, and Antioxidant Activities of an Exopolysaccharide Extracted from Spent Media Wastewater after Leuconostoc mesenteroides WiKim32 Fermentation. ACS Omega 6:8171-8178 (2021). PubMed: 33817476
- D'Onofrio N et al. Phenolic Profiles of Red Wine Relate to Vascular Endothelial Benefits Mediated by SIRT1 and SIRT6. Int J Mol Sci 22:N/A (2021). PubMed: 34073604
- Salahshoor MR et al. Improvement of Petroselinum crispum on Morphine Toxicity in Prefrontal Cortex in Rats. Int J Appl Basic Med Res 10:110-116 (2020). PubMed: 32566527