Free Glycerol Assay Kit II (Colorimetric) (ab155899)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Cell culture supernatant, Plasma, Serum, Suspension cells, Tissue, Tissue Homogenate, Urine
- Sensitivity: 20 µM
Overview
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Product name
Free Glycerol Assay Kit II (Colorimetric)
See all Glycerol kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate -
Assay type
Quantitative -
Sensitivity
< 20 µM -
Range
2 nmol/well - 10 nmol/well -
Assay time
1h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam’s Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.
Detection method: colorimetric – based on enzyme activity
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K634 Free Glycerol Colorimetric Assay Kit II. K634-100 is the same size as the 100 test size of ab155899.
Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Enzyme Mix (lyophilized) Green 1 vial Glycerol Probe Red 1 vial Glycerol Standard Yellow 1 x 0.2ml -
Research areas
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Relevance
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. -
Alternative names
- Glycerin
- Glycerine
Images
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Glycerol Standard CurveGlycerol Standard Curve [a]. Measurement of free glycerol in bovine liver (50 µg), Jurkat cells (40 µg), HeLa cells (30 µg), fish egg (58 µg) & human serum (2 µl) [b]. Assays were performed following kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155899 has been referenced in 1 publication.
- Ma ZG et al. Geniposide Protects against Obesity-Related Cardiac Injury through AMPKa- and Sirt1-Dependent Mechanisms. Oxid Med Cell Longev 2018:6053727 (2018). PubMed: 30533173